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New anti-inflammatory sterols from a gorgonian Pinnigorgia sp
Yin-Di Su1, Ching-Hsiao Cheng2, Zhi-Hong Wen3
1Department of Marine Biotechnology and Resources, National Sun Yat-sen University, Kaohsiung 804, Taiwan; National Museum of Marine Biology and Aquarium, Pingtung 944, Taiwan.
Abstract:
Chemical investigation on the EtOAc-soluble fraction from the MeOH/DCM extract of a gorgonian Pinnigorgia sp. afforded two new sterols, 11-acetoxy-24S-methyl-3β,5α,6α-trihydroxy-9,11-secocholest-7-en-9-one (1) and 5β,6β-epoxy-(22E,24R)-ergosta-8,22-diene-3β,7β-diol (2). The structures of sterols 1 and 2 were elucidated on the basis of spectroscopic analysis and by comparison of their spectroscopic data with those of related analogues. Both 1 and 2 were shown to significantly inhibit the accumulation of the pro-inflammatory iNOS and COX-2 protein in LPS-stimulated RAW264.7 macrophage cells.
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