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Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...

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Related Experiment Video

Updated: Mar 20, 2026

ACT1-CUP1 Assays Determine the Substrate-Specific Sensitivities of Spliceosomal Mutants in Budding Yeast

ACT1-CUP1 Assays Determine the Substrate-Specific Sensitivities of Spliceosomal Mutants in Budding Yeast

Published on: June 30, 2022

Spliceosomal intronogenesis.

Sujin Lee¹, Scott W Stevens²

¹Graduate Program in Cellular and Molecular Biology, The University of Texas at Austin, Austin, TX 78712;

Proceedings of the National Academy of Sciences of the United States of America

|May 25, 2016

Summary

This summary is machine-generated.

Researchers experimentally demonstrated intron transposition, the gain of introns, in yeast. This study provides the first experimental evidence of intron gain, offering insights into genome evolution.

Keywords:

evolution intron spliceosome

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Area of Science:

Molecular Biology
Genomics
Evolutionary Biology

Background:

Introns, intervening sequences in eukaryotic nuclear genomes, are essential for mRNA processing via the spliceosome.
While intron loss is experimentally verified, intron gain mechanisms and evidence have remained largely theoretical, primarily suggested by phylogenetic analyses.
Understanding intron dynamics is crucial for comprehending genome evolution and gene regulation.

Purpose of the Study:

To develop and utilize a strategy for detecting intron gain and loss events experimentally.
To provide the first experimental verification of intron transposition (gain) in any organism.
To investigate the functionality of newly acquired introns and their target genes.

Main Methods:

A screening strategy was employed to detect intron gain and loss events.
Reporter introns were used to track transposition events into specific yeast genes.
Functional analysis of genes with newly acquired introns was performed, including spliceosome-mediated removal and gene expression studies.

Main Results:

The study experimentally verified two instances of intron gain through the transposition of a reporter intron into the yeast genes RPL8B and ADH2.
The newly integrated introns were successfully recognized and removed by the spliceosome from their respective pre-mRNAs.
The RPL8B gene with the new intron (RPL8Bint) was found to be functional when overexpressed in a relevant yeast strain.

Conclusions:

This research presents the first experimental evidence of intron transposition, a novel mechanism for intron gain.
The findings demonstrate that newly acquired introns can be functional and integrated into cellular processes, including splicing.
The study opens new avenues for investigating intron origin, propagation, and their role in shaping eukaryotic genomes.