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Related Concept Videos

Caspases01:24

Caspases

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Caspase, a family of cysteine proteases, serve as effectors in apoptosis. The ced3 gene in C.elegans was first identified to be involved in apoptosis. This gene encodes the ced-3 caspase that is similar to the interleukin-1-beta converting enzyme or ICE in mammals. In addition to apoptosis, caspases also function in the inflammatory response. Inflammatory caspases are essential in activating pro-inflammatory cytokines that recruit immune cells and block the replication of pathogens inside...
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The Extrinsic Apoptotic Pathway01:17

The Extrinsic Apoptotic Pathway

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The extrinsic apoptotic pathway is initiated when extracellular death-inducing signals, such as specific cytokines, activate the death receptors expressed on the cell surface. The immune cells involved in this pathway are natural killer cells (NK cells) and cytotoxic T-lymphocytes. NK cells are critical in innate immune response, while cytotoxic T-lymphocytes are associated with adaptive immune response. These cells recognize specific receptors expressed on the altered cells and activate...
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The Intrinsic Apoptotic Pathway01:31

The Intrinsic Apoptotic Pathway

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Internal cellular stress, such as cellular injury or hypoxia, triggers intrinsic apoptosis. The B-cell lymphoma 2 (Bcl-2) family of proteins are the primary regulators of the intrinsic apoptotic pathway. For example, during DNA damage, checkpoint proteins, such as Ataxia Telangiectasia Mutated (ATM protein) and Checkpoints Factor-2 (Chk2) proteins, are activated. These proteins phosphorylate p53 which further activates pro-apoptotic proteins, such as Bax, Bak, PUMA, and Noxa, and inhibits...
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Apoptosis01:30

Apoptosis

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Apoptosis is a combination of two Greek words, 'apo' and 'ptosis,' meaning separation and falling off, respectively. Hippocrates used this word to describe gangrene, which was caused due to bandaging of fractured bones. Apoptosis was distinguished from necrosis in 1970 when John Kerr reported observations of morphological changes occurring during apoptosis. During one experiment, he observed that the disruption of blood supply to the liver tissue resulted in a size...
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MAPK Signaling Cascades01:07

MAPK Signaling Cascades

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Mitogen-activated protein kinase, or MAPK pathway, activates three sequential kinases to regulate cellular responses such as proliferation, differentiation, survival, and apoptosis. The canonical MAPK pathway starts with a mitogen or growth factor binding to an RTK. The activated RTKs stimulate Ras, which recruits Raf or MAP3 Kinase (MAPKKK), the first kinase of the MAPK signaling cascade. Raf further phosphorylates and activates MEK or MAP2 Kinases (MAPKK), which in turn phosphorylates MAP...
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Autophagic Cell Death01:18

Autophagic Cell Death

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Christian de Duve discovered “autophagy,” a process in which cellular components are engulfed by membrane-bound organelles called autophagosomes. The autophagosomes then fuse with lysosomes to digest the enclosed contents. Autophagy is generally activated in cells to prevent cell death. However, cell death is triggered when the damage is beyond repair.
Autophagy and Apoptosis
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Related Experiment Video

Updated: Mar 20, 2026

Lighting Up the Pathways to Caspase Activation Using Bimolecular Fluorescence Complementation
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Assessing Caspase-1 Activation.

Baptiste Guey1,2,3,4, Virginie Petrilli5,6,7,8,9

  • 1INSERM U1052, Centre de Recherche en Cancérologie de Lyon, Lyon, France.

Methods in Molecular Biology (Clifton, N.J.)
|May 26, 2016
PubMed
Summary

Western blot is a key technique to assess caspase-1 activation, crucial for the innate immune response. This method detects both active and inactive forms of caspase-1 and its substrates.

Keywords:
Caspase-1InflammasomeInterleukin-1beta (IL-1β)Western blot

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Area of Science:

  • Immunology
  • Molecular Biology

Background:

  • Caspase-1 enzymatic activity is vital for innate immunity.
  • It regulates the maturation of pro-inflammatory cytokines IL-1β and IL-18.

Purpose of the Study:

  • To describe the Western blot technique for assessing caspase-1 activation.
  • To highlight the utility of Western blot in studying immune responses.

Main Methods:

  • Western blot analysis.
  • Detection of unprocessed and processed caspase-1.
  • Detection of caspase-1 substrates.

Main Results:

  • Western blot allows for the assessment of caspase-1 activation.
  • The technique enables the simultaneous detection of multiple protein forms.
  • Provides comprehensive data on caspase-1 processing and activity.

Conclusions:

  • Western blot is an effective method for studying caspase-1 activation in the context of innate immunity.
  • This technique offers valuable insights into the regulation of inflammatory cytokines.