Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Flow Cytometry01:23

Flow Cytometry

17.2K
The development of flow cytometry techniques began in 1934 with initial attempts by Andrew Moldavan, a bacteriologist who counted the cells in a flowing capillary system. Moldavan pumped cells through a capillary tube focused under a microscope for visualization. The invention of photometry allowed the measurement of differentially-stained cells, and Louis Kamentsky developed the first multiparameter flow cytometer in 1965 to identify and count the cancer cells in cervical tissue specimens.
In...
17.2K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Deep learning-enabled morphology analysis of bovine sperm for label-free imaging flow cytometry.

Frontiers in veterinary scienceĀ·2026
Same author

Systematic Review: Long-Read Sequencing in Algal Studies.

International journal of molecular sciencesĀ·2026
Same author

Aerobic sister lineage of breviates has gene-rich mitochondrial genomes.

Current biology : CBĀ·2026
Same author

Thermal Management of SSAW Acoustofluidic Devices: Experimental and Numerical Analysis.

Nanomaterials (Basel, Switzerland)Ā·2025
Same author

Sialyl-T Antigen: A Novel Red Blood Cell Determinant for Plasmodium falciparum Invasion.

American journal of hematologyĀ·2025
Same author

Heatwave-Induced Thermal Stratification Shaping Microbial-Algal Communities Under Different Climate Scenarios as Revealed by Long-Read Sequencing and Imaging Flow Cytometry.

ToxinsĀ·2025

Related Experiment Video

Updated: Mar 20, 2026

Microfluidic Imaging Flow Cytometry by Asymmetric-detection Time-stretch Optical Microscopy ATOM
07:19

Microfluidic Imaging Flow Cytometry by Asymmetric-detection Time-stretch Optical Microscopy ATOM

Published on: June 28, 2017

10.8K

Imaging flow cytometry for phytoplankton analysis.

Veronika Dashkova1, Dmitry Malashenkov2, Nicole Poulton3

  • 1Nazarbayev University, Kazakhstan.

Methods (San Diego, Calif.)
|May 26, 2016
PubMed
Summary

Imaging flow cytometry (IFC) offers advanced phytoplankton analysis by combining flow cytometry speed with microscopy imaging. This technology significantly enhances the study of marine microalgae, overcoming limitations of traditional methods.

Keywords:
Imaging flow cytometryMetabolic activityMicroalgaePhytoplanktonViability

More Related Videos

Characterization of Aquatic Biofilms with Flow Cytometry
08:30

Characterization of Aquatic Biofilms with Flow Cytometry

Published on: June 6, 2018

9.8K
Flow Cytometric Analysis of Bimolecular Fluorescence Complementation: A High Throughput Quantitative Method to Study Protein-protein Interaction
11:11

Flow Cytometric Analysis of Bimolecular Fluorescence Complementation: A High Throughput Quantitative Method to Study Protein-protein Interaction

Published on: August 15, 2013

19.0K

Related Experiment Videos

Last Updated: Mar 20, 2026

Microfluidic Imaging Flow Cytometry by Asymmetric-detection Time-stretch Optical Microscopy ATOM
07:19

Microfluidic Imaging Flow Cytometry by Asymmetric-detection Time-stretch Optical Microscopy ATOM

Published on: June 28, 2017

10.8K
Characterization of Aquatic Biofilms with Flow Cytometry
08:30

Characterization of Aquatic Biofilms with Flow Cytometry

Published on: June 6, 2018

9.8K
Flow Cytometric Analysis of Bimolecular Fluorescence Complementation: A High Throughput Quantitative Method to Study Protein-protein Interaction
11:11

Flow Cytometric Analysis of Bimolecular Fluorescence Complementation: A High Throughput Quantitative Method to Study Protein-protein Interaction

Published on: August 15, 2013

19.0K

Area of Science:

  • Marine Biology
  • Analytical Chemistry
  • Biotechnology

Background:

  • Traditional phytoplankton analysis methods have limitations in speed and detail.
  • Imaging flow cytometry (IFC) integrates flow cytometry and microscopy for enhanced cell analysis.
  • IFC is rapidly advancing as a powerful cell imaging platform for microalgal research.

Purpose of the Study:

  • To review the concepts and instrumentation of IFC for phytoplankton analysis.
  • To highlight IFC's applications in assessing phytoplankton communities, including composition, abundance, and size structure.
  • To present data on viability and metabolic assessment of cyanobacteria using IFC.

Main Methods:

  • Review of existing literature on IFC instrumentation and applications in phytoplankton research.
  • Presentation of data acquired using the Imagestream X Mark II imaging cytometer.
  • Analysis of phytoplankton community composition, abundance, size structure, biovolume, and metabolic activity.

Main Results:

  • IFC overcomes limitations of conventional techniques, significantly advancing phytoplankton analysis.
  • IFC enables detailed assessment of complex phytoplankton communities and harmful algal bloom species.
  • Demonstrated IFC's utility in evaluating the viability and metabolic activity of cyanobacteria.

Conclusions:

  • IFC possesses immense potential for microalgal research, offering unprecedented analytical capabilities.
  • Further developments in IFC technology are expected to expand its applications in marine science.
  • IFC provides a robust platform for understanding phytoplankton dynamics and ecological roles.