Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

RNA Polymerase II Accessory Proteins02:36

RNA Polymerase II Accessory Proteins

11.3K
Proteins that regulate transcription can do so either via direct contact with RNA Polymerase or through indirect interactions facilitated by adaptors, mediators, histone-modifying proteins, and nucleosome remodelers. Direct interactions to activate transcription is seen in bacteria as well as in some eukaryotic genes. In these cases, upstream activation sequences are adjacent to the promoters, and the activator proteins interact directly with the transcriptional machinery. For example, in...
11.3K
Transcription Factors02:16

Transcription Factors

83.7K
Tissue-specific transcription factors contribute to diverse cellular functions in mammals. For example, the gene for beta globin, a major component of hemoglobin, is present in all cells of the body. However, it is only expressed in red blood cells because the transcription factors that can bind to the promoter sequences of the beta globin gene are only expressed in these cells. Tissue-specific transcription factors also ensure that mutations in these factors may impair only the function of...
83.7K
Transcription Factors02:16

Transcription Factors

26.6K
26.6K
Eukaryotic Transcription Activators02:42

Eukaryotic Transcription Activators

13.2K
Transcription activators are proteins that promote the transcription of genes from DNA to RNA. In most cases, these proteins contain two separate domains ‒ a domain that binds to DNA and a domain for activating transcription; however, in some cases, a single domain is responsible for both binding and activation of transcription, as seen in the glucocorticoid receptor and MyoD.
The binding domains are capable of recognizing and interacting with regulatory sequences on the DNA. These...
13.2K
General Transcription Factors01:30

General Transcription Factors

7.5K
Tissue-specific transcription factors contribute to diverse cellular functions in mammals. For example, the gene for beta globin, a major component of hemoglobin, is present in all cells of the body. However, it is only expressed in red blood cells because the transcription factors that can bind to the promoter sequences of the beta globin gene are only expressed in these cells. Tissue-specific transcription factors also ensure that mutations in these factors may impair only the function of...
7.5K
Genome Annotation and Assembly03:36

Genome Annotation and Assembly

21.4K
The genome refers to all of the genetic material in an organism. It can range from a few million base pairs in microbial cells to several billion base pairs in many eukaryotic organisms. Genome assembly refers to the process of taking the DNA sequencing data and putting it all back together in a correct order to create a close representation of the original genome. This is followed by the identification of functional elements on the newly assembled genome, a process called genome annotation.
21.4K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

KOLF2.1J iTF-Microglia: A standardized platform to study microglial transcriptional regulatory networks in CNS disease.

iScience·2026
Same author

IRS4 is a PI3K-activating cancer dependency up-regulated through DNA rearrangements or epigenetic mechanisms in multiple solid tumors.

Science advances·2026
Same author

Rapid and repeated evolution of myosin copy number in threespine stickleback.

Current biology : CB·2026
Same author

Rapid and repeated evolution of myosin copy number in threespine stickleback.

bioRxiv : the preprint server for biology·2026
Same author

Domain mapping of disease mutations reveals pathogenic SORL1 variants in Alzheimer's disease.

Molecular neurodegeneration·2025
Same author

Development of a Dynamic Counterfactual Risk Stratification Strategy for Newly Diagnosed Patients With AML Treated With Venetoclax and Azacitidine.

JCO clinical cancer informatics·2025
Same journal

Editorial for special issue "When should mathematical models be used in biology".

Seminars in cell & developmental biology·2026
Same journal

Conserved machinery, divergent functions: evolutionary plasticity of the STK36/ULK4 kinase complex in ciliogenesis and signaling.

Seminars in cell & developmental biology·2026
Same journal

Chemical biology tools for studying tissue development.

Seminars in cell & developmental biology·2026
Same journal

Tetrahymena as a model organism for cilia research.

Seminars in cell & developmental biology·2026
Same journal

Emerging Concepts in Cardiovascular Development and Regeneration.

Seminars in cell & developmental biology·2026
Same journal

Endothelial origin of hematopoietic stem cells: Insights from new technologies and unresolved questions.

Seminars in cell & developmental biology·2026
See all related articles

Related Experiment Video

Updated: Mar 20, 2026

A Web-Based Workflow for Selecting Gene- and Tissue-Specific Enhancers
08:12

A Web-Based Workflow for Selecting Gene- and Tissue-Specific Enhancers

Published on: July 18, 2025

782

Decoding transcriptional enhancers: Evolving from annotation to functional interpretation.

Krysta L Engel1, Mark Mackiewicz1, Andrew A Hardigan2

  • 1HudsonAlpha Institute for Biotechnology, Huntsville, AL 35806, United States.

Seminars in Cell & Developmental Biology
|May 26, 2016
PubMed
Summary
This summary is machine-generated.

Gene regulation is key to development and disease. Enhancer elements play a crucial role, and new genome editing tools allow for systematic, high-throughput functional evaluation of these regulatory elements.

Keywords:
CRISPR genome editingDevelopmentDiseaseEnhancersFunctional genomicsNext-generation sequencingTranscription factors

More Related Videos

A Computational Pipeline for Intergenic/Intragenic Enhancer RNA Quantification in Mouse Embryonic Stem Cells
06:02

A Computational Pipeline for Intergenic/Intragenic Enhancer RNA Quantification in Mouse Embryonic Stem Cells

Published on: October 28, 2025

651
Author Spotlight: An Integrated Workflow to Study the Promoter-Centric Spatio-Temporal Genome Architecture in Scarce Cell Populations
11:36

Author Spotlight: An Integrated Workflow to Study the Promoter-Centric Spatio-Temporal Genome Architecture in Scarce Cell Populations

Published on: April 21, 2023

3.1K

Related Experiment Videos

Last Updated: Mar 20, 2026

A Web-Based Workflow for Selecting Gene- and Tissue-Specific Enhancers
08:12

A Web-Based Workflow for Selecting Gene- and Tissue-Specific Enhancers

Published on: July 18, 2025

782
A Computational Pipeline for Intergenic/Intragenic Enhancer RNA Quantification in Mouse Embryonic Stem Cells
06:02

A Computational Pipeline for Intergenic/Intragenic Enhancer RNA Quantification in Mouse Embryonic Stem Cells

Published on: October 28, 2025

651
Author Spotlight: An Integrated Workflow to Study the Promoter-Centric Spatio-Temporal Genome Architecture in Scarce Cell Populations
11:36

Author Spotlight: An Integrated Workflow to Study the Promoter-Centric Spatio-Temporal Genome Architecture in Scarce Cell Populations

Published on: April 21, 2023

3.1K

Area of Science:

  • Genetics
  • Molecular Biology
  • Genomics

Background:

  • Differential gene expression across cell types is fundamental for multicellular life and complex cellular functions.
  • Understanding gene regulation is critical for elucidating developmental processes, evolutionary principles, and disease mechanisms.

Purpose of the Study:

  • To review the current understanding of gene regulation, focusing on the role of enhancer elements.
  • To discuss the function of transcriptional enhancers in development and disease.
  • To catalogue experimental tools for studying gene regulatory elements and highlight novel techniques.

Main Methods:

  • Review of existing literature on gene regulation and enhancer function.
  • Discussion of advances in functional genomic methods and genome editing technologies.
  • Cataloguing of experimental tools for studying transcriptional regulatory elements.

Main Results:

  • Genome editing technologies enable systematic, high-throughput functional evaluation of enhancers and other transcriptional regulatory elements.
  • Functional genomic methods have advanced gene regulation studies but often lack functional interpretation.
  • Novel techniques are crucial for deciphering the complex gene regulatory landscape.

Conclusions:

  • Transcriptional enhancers are critical regulatory elements with significant roles in development and disease.
  • Innovations in genome editing are transforming the study of gene regulation, enabling coordinated, high-throughput functional assessments.
  • Future research utilizing novel techniques will further elucidate the complex gene regulatory landscape.