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Updated: Mar 20, 2026
Author Spotlight: Quantification of Complex Lipidomic Samples Using Stable Isotope Labeling
Published on: August 23, 2024
An LC-MRM method for measuring intestinal triglyceride assembly using an oral stable isotope-labeled fat challenge
Xiaofang Li1, Elizabeth J Parks2, David G McLaren1
1Merck Research Laboratories, Merck & Co., Inc., Kenilworth, NJ, USA.
Aim:
A traditional oral fatty acid challenge assesses absorption of triacylglycerol (TG) into the periphery through the intestines, but cannot distinguish the composition or source of fatty acid in the TG. Stable isotope-labeled tracers combined with LC-MRM can be used to identify and distinguish TG synthesized with dietary and stored fatty acids.
Results:
Concentrations of three abundant TGs (52:2, 54:3 and 54:4) were monitored for incorporation of one or two (2)H11-oleate molecules per TG. This method was subjected to routine assay validation and meets typical requirements for an assay to be used to support clinical studies.
Conclusion:
Calculations for the fractional appearance rate of TG in plasma are presented along with the intracellular enterocyte precursor pool for 12 study participants.
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