Gonad development and hermaphroditism in the ascidian Botryllus schlosseri

The colonial ascidian Botryllus schlosseri is an ideal model organism for studying gonad development and hermaphroditism. B. schlosseri has been reared in laboratories for over half a century, and its unique biology allows investigators to probe the processes of germ cell migration and gonad formation, resorption, and regeneration. Following metamorphosis, colonies of B. schlosseri show a synchronized and sequential fertility program that, under standard laboratory conditions, begins with a juvenile stage with no visible gonads and subsequently develops testes at 9 weeks followed later by the production of oocytes-thus resulting in hermaphroditic individuals. The timing of oocyte production varies according to the season, and adult B. schlosseri colonies can cycle among infertile and both male and hermaphrodite fertile states in response to changing environmental conditions. Thus, these acidians are amenable to studying the molecular mechanisms controlling fertility, and recent genomic and transcriptomic databases are providing insight to the key genes involved. Here, we review the techniques and approaches developed to study germ cell migration and gonad formation in B. schlosseri, and include novel videos showing processes related to oocyte ovulation and sperm discharge. In the future, this valuable invertebrate model system may help understand the mechanisms of gonad development and regeneration in a chordate. Mol. Reprod. Dev. 84: 158-170, 2017. © 2016 Wiley Periodicals, Inc.