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A nanoplasmonic probe as a triple channel colorimetric sensor array for protein discrimination
Jinpeng Mao1, Yuexiang Lu2, Ning Chang1
1Department of Chemistry, Capital Normal University, Xisanhuan North Rd. 105, Beijing, 100048, P. R. China. yueyingliu@cnu.edu.cn.
The Analyst
|May 28, 2016
Summary
Protein interactions with gold nanoparticles (AuNPs) and DNA conjugates alter their behavior, creating unique color changes for protein identification. This method allows for naked-eye discrimination of different proteins.
Area of Science:
- Nanotechnology
- Biochemistry
- Analytical Chemistry
Background:
- Gold nanoparticles (AuNPs) and DNA conjugates exhibit specific aggregation and self-assembly behaviors.
- Protein interactions can influence these nanomaterial behaviors, leading to observable changes.
Purpose of the Study:
- To investigate how protein interactions modify the salt-induced aggregation, nanoparticle regrowth, and self-assembly of AuNPs and DNA conjugates.
- To develop a colorimetric method for protein discrimination based on these altered behaviors.
Main Methods:
- Utilizing gold nanoparticles (AuNPs) and DNA conjugates.
- Inducing salt-dependent aggregation and observing nanoparticle regrowth and self-assembly.
- Analyzing color changes and unique fingerprint patterns generated upon protein interaction.
- Employing triple-channel colorimetric signals for analysis.
Main Results:
- Protein interactions significantly altered the aggregation, regrowth, and self-assembly dynamics of AuNPs and DNA conjugates.
- Distinct color changes and unique fingerprint patterns were observed for different proteins.
- Triple-channel colorimetric signals effectively differentiated various proteins.
Conclusions:
- The study demonstrates a novel colorimetric approach for protein discrimination using AuNPs and DNA conjugates.
- Protein-induced changes in nanomaterial behavior provide a basis for sensitive and visually detectable protein identification.
- This method offers a simple, naked-eye technique for analyzing protein fingerprints.
