Functionalized nanoparticles based solid-phase membrane micro-tip extraction and high-performance liquid...

Area of Science:

• Green Chemistry and Nanotechnology
• Analytical Chemistry and Separation Science
• Biochemical Analysis

Background:

• Traditional methods for vitamin B complex analysis can be time-consuming and costly.
• Development of efficient and cost-effective sample preparation techniques is crucial for accurate biochemical analysis.
• Nanomaterials offer unique properties for advanced separation applications.

Purpose of the Study:

• To synthesize and characterize novel iron nanoparticles functionalized with 1-butyl-3-methylimidazolium bromide.
• To develop a solid-phase membrane micro-tip extraction method for vitamin B complex from plasma.
• To establish a robust high-performance liquid chromatography (HPLC) method for vitamin B complex quantification.

Main Methods:

• Green synthesis of iron nanoparticles followed by functionalization with 1-butyl-3-methylimidazolium bromide.
• Characterization using FTIR, EDX, XRD, SEM, and TEM.
• Optimization of solid-phase membrane micro-tip extraction parameters (sorbent amount, time, pH, solvent, volume, desorption time).
• Development of an HPLC method with a PhE column and optimized mobile phase composition.

Main Results:

• Successfully synthesized and characterized functionalized iron nanoparticles.
• Optimized extraction method achieved 60-83% recovery for all eight vitamin B complex components.
• HPLC method demonstrated good separation factors (capacity, separation, resolution) and selectivity.
• The developed method is fast, selective, inexpensive, economic, and reproducible.

Conclusions:

• The developed green synthesis and extraction method using functionalized iron nanoparticles is effective for vitamin B complex isolation from plasma.
• The combined sample preparation and HPLC method provide a reliable approach for analyzing vitamin B complex in biological and environmental samples.
• This study highlights the potential of functionalized nanomaterials in advancing analytical chemistry techniques.