A high-resolution method to assess cell multinucleation with cytoplasm-localized fluorescent probes
Hui Wen1, Qinghua Cui2, Hui Meng2
1State Key Laboratory of Bioactive Substances and Function of Natural Medicine, Institute of Materia Medica, Peking Union Medical College and Chinese Academy of Medical Sciences, Beijing, 100050, China. hcui@imm.ac.cn yindali@imm.ac.cn and Beijing Key Laboratory of Active Substances Discovery and Drugability Evaluation, Institute of Materia Medica, Peking Union Medical College and Chinese Academy of Medical Sciences, Beijing, 100050, China.
We developed a simple cytoplasm-localized fluorescent probe (CLFP) method to accurately assess cell multinucleation and nuclear fragments, offering superior results to traditional nuclear staining techniques.
Area of Science:
- Cell biology
- Genetics
- Biotechnology
Background:
- Cell multinucleation is a significant indicator of chromosomal instability.
- Conventional methods for assessing multinucleation often rely on nuclear staining, which can be less accurate.
Purpose of the Study:
- To introduce a novel and improved method for quantifying cell multinucleation.
- To demonstrate the advantages of cytoplasm-localized fluorescent probes (CLFP) over traditional nuclear staining.
Main Methods:
- Development of a cytoplasm-localized fluorescent probe (CLFP) assay.
- Utilizing CLFP for high-resolution imaging of cell multinucleation.
- Accurate calculation of nuclear fragments using CLFP imaging.
Main Results:
- The CLFP method provides superior assessment of cell multinucleation compared to conventional methods.
- High-resolution imaging enabled precise counting of nuclear fragments.
- The method is simple and convenient for researchers.
Conclusions:
- CLFP is an effective and superior tool for assessing cell multinucleation.
- This method facilitates accurate measurement of nuclear fragments, aiding in the study of chromosomal instability.
