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Updated: Mar 20, 2026

A Guide to Structured Illumination TIRF Microscopy at High Speed with Multiple Colors
Published on: May 30, 2016
Structured illumination temporal compressive microscopy
Abstract:
We present a compressive video microscope based on structured illumination with incoherent light source. The source-side illumination coding scheme allows the emission photons being collected by the full aperture of the microscope objective, and thus is suitable for the fluorescence readout mode. A 2-step iterative reconstruction algorithm, termed BWISE, has been developed to address the mismatch between the illumination pattern size and the detector pixel size. Image sequences with a temporal compression ratio of 4:1 were demonstrated.
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