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Neutralization Assay for Chikungunya Virus Infection: Plaque Reduction Neutralization Test

Nor Azila Muhammad Azami1,2, Meng Ling Moi3,4, Tomohiko Takasaki1

  • 1Department of Virology 1, National Institute of Infectious Diseases, Toyama 1-23-1 Shinjuku-ku, Tokyo, 162-8640, Japan.

Methods in Molecular Biology (Clifton, N.J.)
|May 29, 2016
PubMed
Summary

This study details a plaque reduction neutralization assay (PRNT) for quantifying Chikungunya virus (CHIKV) neutralizing antibodies. The method uses Vero cells to determine antibody titers by measuring the reduction in viral activity.

Keywords:
AntibodyChikungunyaNeutralizing antibodyNeutralizing assayPlaque reduction neutralization testVero cell lines

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