In vitro interaction between gingival fibroblasts and various root surfaces

The objective of the present in vitro study was to examine the effect of various root surface treatments on the initial attachment of gingival fibroblasts. Cultured fibroblasts were well spread and attached to demineralized dentin with citric acid, superficially curetted cementum, and root surface with periodontal ligament, while those on the planed dentin showed a poorly spread appearance. The first three root surfaces exhibited rough and irregular surface appearances with a well-developed filopodia and the latter root surface exhibited a smooth appearance, suggesting the texture of the root surface is closely associated with cell attachment. Interiorized collagen fibrils and numerous cytoplasmic vesicles were seen along the cell membrane of fibroblasts facing the root surfaces of the planed dentin, demineralized dentin, and superficially curetted cementum. Thus even the artificially exposed tooth surface with citric acid may be subjected to further alteration by fibroblasts.