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A candidate reference method for the determination of magnesium in serum
W R Külpmann1, D Ruschke, J Büttner
1Institut für Klinische Chemie, Medizinische Hochschule Hannover, Federal Republic of Germany.
Journal of Clinical Chemistry and Clinical Biochemistry. Zeitschrift Fur Klinische Chemie Und Klinische Biochemie
|January 1, 1989
Summary
A new flame atomic absorption spectrometry method accurately measures serum magnesium. This validated reference method demonstrates high precision and reliability for clinical diagnostics.
Area of Science:
- Clinical Chemistry
- Analytical Chemistry
- Spectroscopy
Background:
- Accurate determination of serum magnesium is crucial for clinical diagnostics.
- Existing reference methods require validation for reliability and precision.
Purpose of the Study:
- To develop and validate a candidate reference method for serum magnesium determination.
- To assess the precision, accuracy, and reliability of the proposed method.
Main Methods:
- Flame atomic absorption spectrometry (FAAS) was employed.
- Method validation included analysis of standard solutions and assessment of precision using replicates.
- Comparison with definitive values was performed.
Main Results:
- The candidate reference method demonstrated high precision with relative standard deviations ranging from 0.18 to 1.07%.
- Deviations from definitive values were minimal (-0.15% to 0.44%), indicating excellent accuracy.
- The method's performance was comparable to established reference methods for other electrolytes.
Conclusions:
- The developed FAAS method is a reliable and accurate reference method for serum magnesium determination.
- The method exhibits suitable precision and accuracy for clinical laboratory use.
- Further transferability studies are recommended to confirm its widespread applicability.