Related Experiment Video For Complement factor
Updated: Mar 20, 2026
Preparation of Synaptic Plasma Membrane and Postsynaptic Density Proteins Using a Discontinuous Sucrose Gradient
Published on: September 3, 2014
Complement factors alter the amount of PrP(Sc) in primary-cultured mouse cortical neurons associated with increased
Rie Hasebe1, Misaki Tanaka1, Akio Suzuki1
1Laboratory of Veterinary Hygiene, Graduate School of Veterinary Medicine, Hokkaido University, Nishi 9, Kita 18, Kita-ku, Sapporo 060-0818, Japan.
Abstract:
We examined the effects of complement factors on primary-cultured neurons infected with prions. The amount of protease K (PK)-resistant abnormal form of prion protein (PrP(Sc)) reached a maximum level at 12 and 16 days post exposure (dpe) in 22L- and Chandler-infected neurons, respectively. In Chandler-infected neurons, the reaction of complement factors C1q, C3 and C9 significantly increased membrane permeability. This was followed by a decrease of PK-resistant PrP(Sc) at 16 and 20dpe. In contrast, in 22L-infected neurons, the effects of complement factors were observed at 12 and 16dpe, but not at 20dpe. Membrane permeability also increased in 22L-infected neurons by reaction of complement factor C3, but interestingly, the amount of PK-resistant PrP(Sc) initially decreased, and then increased. These results suggest that the reactivity of complement factors in prion-infected neurons depends on the amount of PrP(Sc) and the prion strain.
