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Updated: Mar 20, 2026

Microbiota Analysis Using Two-step PCR and Next-generation 16S rRNA Gene Sequencing
Published on: October 15, 2019
Gut microbiota and inflammatory joint diseases.
1Service de Rhumatologie, Hôpital Ambroise-Paré (AP-HP), 9, avenue Charles-de-Gaulle, 92100 Boulogne-Billancourt, France; Laboratoire d'Excellence, Université Paris Diderot, Sorbonne Paris Cité, 75013 Paris, France; INSERM U1173, UFR des Sciences de la santé Simone-Veil, Université de Versailles-Saint Quentin, 2, avenue de la source de la Bièvre, 78180 Montigny-le-Bretonneux, France.
Changes in the gut microbiota are linked to chronic inflammatory diseases like rheumatoid arthritis. Understanding these microbial shifts may lead to new treatments and biomarkers for inflammatory conditions.
Area of Science:
- Microbiome research
- Immunology
- Gastroenterology
Background:
- The etiology of most chronic inflammatory diseases remains unknown, with chronic infection being a leading hypothesis.
- Advances in technology enable comprehensive analysis of the gut microbiota, comprising unculturable microorganisms.
- Inflammatory bowel disease (IBD) is characterized by persistent gut microbiota alterations that may contribute to its pathogenesis.
Purpose of the Study:
- To investigate the association between gut microbiota and joint inflammation in human patients.
- To explore alterations in the gut microbiota of patients with rheumatoid arthritis, psoriatic arthritis, and spondyloarthritis.
- To compare gut microbiota changes in these conditions with those observed in IBD.
Main Methods:
- Analysis of gut microbiota composition in patients with inflammatory joint diseases.
- Comparison of microbial profiles across different diagnoses (rheumatoid arthritis, psoriatic arthritis, spondyloarthritis) and with IBD patients.
- Assessment of microbial diversity and specific bacterial group frequencies.
Main Results:
- Substantial alterations in gut microbiota were observed in patients with rheumatoid arthritis, psoriatic arthritis, and spondyloarthritis.
- Microbiota changes differed across these diagnoses and studies.
- Observed alterations included decreased microbial diversity and reduced frequencies of immunoregulatory Firmicutes, similar to IBD findings.
Conclusions:
- Gut microbiota alterations are present in patients with inflammatory arthritis, mirroring some changes seen in IBD.
- These findings suggest a potential role for the gut microbiota in the pathogenesis of joint inflammation.
- The study highlights new avenues for understanding inflammatory diseases and developing novel biomarkers and therapies.
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