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Bacteriophage Effectiveness for Biocontrol of Foodborne Pathogens Evaluated via High-Throughput Settings
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A Newly Isolated Bacteriophage, PBES 02, Infecting Cronobacter sakazakii

Hyung Ju Lee1, Wan Il Kim1, Young Chan Kwon1

  • 1Hana High School, Seoul 03305, Republic of Korea.

Journal of Microbiology and Biotechnology
|May 31, 2016
PubMed

Insights

A novel bacteriophage, PBES 02, was isolated and characterized for its potential to control Cronobacter sakazakii contamination in infant formula. This phage demonstrated stability across a range of temperatures and pH levels, effectively reducing bacterial presence.

Area of Science:

  • Microbiology
  • Virology
  • Food Safety

Background:

  • Cronobacter sakazakii is a foodborne pathogen that can contaminate infant formula.
  • Bacteriophages are viruses that infect bacteria and show promise as a biocontrol agent.

Purpose of the Study:

  • To isolate and characterize a novel bacteriophage, PBES 02, targeting Cronobacter sakazakii.
  • To evaluate the stability and efficacy of PBES 02 as a biocontrol agent for Cronobacter sakazakii in infant formula.

Main Methods:

  • Isolation and characterization of bacteriophage PBES 02 using transmission electron microscopy.
  • Determination of phage morphology, latent period, burst size, and stability under various temperature and pH conditions.
  • Genome sequencing and analysis of PBES 02, including open reading frame (ORF) annotation and comparison with related phages.

Main Results:

  • PBES 02, a Myoviridae family phage with a 90 nm head and 130 nm tail, was isolated.
  • The phage exhibited a latent period of 30 min and a burst size of 250, remaining infective between 4°C-55°C and pH 6-10.
  • PBES 02 effectively reduced Cronobacter sakazakii in infant formula broth and possesses a double-stranded DNA genome with 299 ORFs.

Conclusions:

  • Bacteriophage PBES 02 is a promising biocontrol agent against Cronobacter sakazakii.
  • Its stability and efficacy in reducing bacterial contamination highlight its potential application in food safety, particularly for infant formula.
  • Genomic analysis reveals PBES 02 is related to other Cronobacter sakazakii phages, suggesting a broad distribution of these agents.
Keywords:
Bacteriophage PBES 02Cronobacter sakazakiigenomic analysisphage therapy

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