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Updated: Mar 20, 2026

Measurement of Tissue Non-Heme Iron Content using a Bathophenanthroline-Based Colorimetric Assay
Published on: January 31, 2022
Rapid assessment of iron in blood plasma and serum by spectrophotometry with cloud-point extraction
Tatyana Samarina1, Mikhail Proskurnin1
1Chemistry Department, Agilent Technologies Partner Laboratory - Analytical Centre, M.V. Lomonosov Moscow State University, Moscow, 119991, Russian Federation.
Abstract:
Rapid photometric assessment of iron in blood plasma and serum by a simple procedure after the extraction of iron(II) complex with 1-nitroso-2-naphthol in the micellar phase of a nonionic surfactant at the cloud point upon heating (pH range is 4.5-6.3) is proposed. The procedure trueness was verified using a standard reference protocol using bathophenanthroline. The advantages of the procedure are higher sensitivity than the reference protocol: the limit of detection is 0.03 μg/mL, the limit of quantitation is 0.1 μg/mL, the determination range is 0.1 - 2.8 μg/mL (RSD 0.02-0.10). Copper does not interfere with the iron assessment.
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