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Related Experiment Video

Updated: Mar 20, 2026

Dry Film Photoresist-based Electrochemical Microfluidic Biosensor Platform: Device Fabrication, On-chip Assay Preparation, and System Operation
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Pt@AuNPs integrated quantitative capillary-based biosensors for point-of-care testing application.

Ze Wu1, Qiangqiang Fu1, Shiting Yu1

  • 1Department of Bioengineering, Guangdong Province Key Laboratory of Molecular Immunology and Antibody Engineering, Jinan University, Guangzhou 510632, PR China.

Biosensors & Bioelectronics
|May 31, 2016
PubMed
Summary

A new portable, cost-effective capillary biosensor enables naked-eye detection of biomarkers like prostate-specific antigen (PSA) and carcinoembryonic antigen (CEA) for point-of-care testing.

Keywords:
Capillary-based biosensorCarcinoembryonicantigenCore-shell Pt@Au nanoparticlePoint-of-care testingProstate-specific antigen

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Area of Science:

  • Nanotechnology
  • Biomedical Engineering
  • Analytical Chemistry

Background:

  • Current diagnostic tools are often bulky and expensive, limiting their use in point-of-care settings.
  • There is a significant need for portable, cost-effective diagnostic solutions for rapid biomarker detection.
  • Developing simple, visual detection methods is crucial for accessible healthcare.

Purpose of the Study:

  • To develop and validate a cost-effective, portable capillary-based biosensor for quantitative biomarker detection.
  • To demonstrate the biosensor's capability for naked-eye readout using visual cues.
  • To assess the biosensor's performance in detecting cancer biomarkers like PSA and CEA.

Main Methods:

  • Utilized core-shell Pt@Au nanoparticles (Pt@AuNPs) as catalysts to decompose hydrogen peroxide (H2O2), producing oxygen (O2).
  • Developed a capillary-based biosensor where O2 production, indicated by blue ink movement, correlates with biomarker concentration.
  • Constructed sandwich immunoassays by linking Pt@AuNPs with antibodies for specific protein capture.

Main Results:

  • The distance of ink movement in the capillary was directly proportional to the concentration of target proteins.
  • Achieved a linear detection range (LDR) of 0.02–2.5 ng/mL for prostate-specific antigen (PSA) with a limit of detection (LOD) of 0.017 ng/mL.
  • Demonstrated an LDR of 0.063–16 ng/mL for carcinoembryonic antigen (CEA) with an LOD of 0.044 ng/mL.
  • Results from clinical serum samples showed good correlation with established chemiluminescence immunoassays (CLIAs).

Conclusions:

  • The developed capillary-based biosensor offers a cost-effective and portable solution for quantitative biomarker detection.
  • The naked-eye readout mechanism simplifies the diagnostic process, making it suitable for point-of-care applications.
  • This biosensor technology shows promise for accessible and rapid disease screening, particularly for cancer biomarkers.