Related Experiment Video
Updated: Mar 20, 2026
Isolation and Time-Lapse Imaging of Primary Mouse Embryonic Palatal Mesenchyme Cells to Analyze Collective Movement Attributes
Published on: February 13, 2021
Spatio-temporal expression of Sox genes in murine palatogenesis
Momoko Watanabe1, Katsushige Kawasaki2, Maiko Kawasaki3
1Division of Oral Anatomy, Department of Oral Biological Science, Niigata University, Graduate School of Medical and Dental Sciences, Niigata, Japan; Division of Oral and Maxillofacial Surgery, Department of Health Science, Niigata University, Graduate School of Medical and Dental Sciences, Niigata, Japan.
Abstract:
Members of the Sox gene family play critical roles in many biological processes including organogenesis. We carried out comparative in situ hybridisation analysis of seventeen Sox genes (Sox1-14, 17, 18 and 21) during murine palatogenesis from initiation to fusion of the palatal shelves above the dorsal side of the tongue. At palatal shelf initiation (E12.5), the localized expression of six Sox genes (Sox2, 5, 6, 9, 12 and 13) was observed in the shelves, whereas Sox4 and Sox11 showed ubiquitious expression. During the down-growth of palatal shelves (E13.5), Sox4, Sox5, and Sox9 exhibited restricted expression to the interior side of the palatal shelves facing the tongue. Following elevation of the palatal shelves (E14.5), Sox2, Sox11 and Sox21 expression was present in the midline epithelial seam. We thus identify dynamic spatio-temporal expression of Sox gene family during the process of palatogenesis.
