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Related Concept Videos

MicroRNAs01:22

MicroRNAs

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MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...
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MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After...
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pre-mRNA Processing02:01

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In eukaryotic cells, transcripts made by RNA polymerase are modified and processed before exiting the nucleus. Unprocessed RNA is called precursor mRNA or pre-mRNA to distinguish it from mature mRNA.
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Mitochondrial Precursor Proteins01:39

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Mitochondrial precursors are partially unfolded or loosely folded polypeptide chains. Newly synthesized precursors are inhibited from spontaneously folding into their native conformation by the cytosolic chaperones, heat shock proteins 70 (Hsp70), and mitochondrial import stimulation factors (MSFs). Precursors bound to MSFs are guided to the TOM70-TOM37 receptors, while precursors bound to Hsp70  chaperones are targetted to TOM20-TOM22 receptor complexes.
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Related Experiment Video

Updated: Mar 20, 2026

mirMachine: A One-Stop Shop for Plant miRNA Annotation
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miRNAFold: a web server for fast miRNA precursor prediction in genomes.

Christophe Tav1, Sébastien Tempel1, Laurent Poligny1

  • 1IBISC-IBGBI, Université Evry, Genopole, 23 Boulevard de France, 91037 Evry CEDEX, France.

Nucleic Acids Research
|June 1, 2016
PubMed
Summary

This study introduces miRNAFold, a fast and sensitive computational tool for identifying novel microRNA precursors. The user-friendly web server enables large-scale genome analysis for miRNA discovery.

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Area of Science:

  • Bioinformatics
  • Genomics
  • Molecular Biology

Background:

  • Non-coding RNAs (ncRNAs) play crucial roles in biological processes, particularly post-transcriptionally.
  • MicroRNAs (miRNAs) are a significant class of ncRNAs requiring efficient identification tools.
  • Ab initio prediction methods are essential for discovering novel miRNAs.

Purpose of the Study:

  • To present a web server for large-scale identification of miRNA precursors.
  • To introduce the miRNAFold algorithm for rapid and sensitive prediction of miRNA hairpin structures.

Main Methods:

  • Development of the miRNAFold algorithm for predicting miRNA hairpin structures.
  • Implementation of miRNAFold as a user-friendly web server and standalone tool.
  • Application of the tool for large-scale genomic analysis.

Main Results:

  • miRNAFold demonstrates high sensitivity in predicting miRNA hairpin structures.
  • The algorithm enables fast identification of miRNA precursors.
  • The web server facilitates efficient, large-scale miRNA identification.

Conclusions:

  • The miRNAFold web server provides an efficient and sensitive solution for novel miRNA precursor identification.
  • The tool supports large-scale genomic analysis, aiding miRNA discovery.
  • Accessible via a web server and standalone version, miRNAFold empowers biological research.