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Late stage definitive endodermal differentiation can be defined by Daf1 expression.

Soichiro Ogaki1,2,3, Hisayoshi Omori2, Mayu Morooka2

  • 1School of Life Science and Technology, Tokyo Institute of Technology, 4259-B-25 Nagatsuta-cho, Midori-ku, Yokohama, Kanagawa, 226-8501, Japan.

BMC Developmental Biology
|June 2, 2016
PubMed
Summary
This summary is machine-generated.

Decay accelerating factor 1 (Daf1) marks late definitive endoderm (DE) cells. These Daf1-expressing cells exhibit reduced proliferation and adhesion, distinguishing them from early DE.

Keywords:
AdhesionDaf1Definitive endodermIn vitro differentiationPluripotent stem cellProliferation

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Area of Science:

  • Developmental biology
  • Stem cell research
  • Cell differentiation

Background:

  • Definitive endoderm (DE) forms the respiratory and digestive systems.
  • Sox17 and Cxcr4 are established DE markers.
  • Decay accelerating factor 1 (Daf1/CD55) was identified as a novel DE marker in mouse embryonic stem cells (ESCs).

Purpose of the Study:

  • To characterize Daf1-expressing cells during definitive endoderm differentiation.
  • To investigate the proliferative and adhesive properties of Daf1+ DE cells.

Main Methods:

  • Utilized an ESC differentiation system.
  • Analyzed Daf1 expression to distinguish early and late DE.
  • Examined proliferative and cell matrix adhesive characteristics of Daf1+ DE cells.
  • Purified SOX17(low) early DE cells for further analysis.

Main Results:

  • Daf1 expression differentiates early (Daf1-) from late (Daf1+) DE.
  • Daf1+ late DE cells display low proliferation rates.
  • Daf1+ late DE cells exhibit low cell matrix adhesive capacity.
  • SOX17(low) early DE cells differentiate into Daf1+ Sox17(high) late DE cells.

Conclusions:

  • Daf1 marks a distinct population of late definitive endoderm cells.
  • These late DE cells are characterized by slow proliferation and low adhesion.
  • Daf1 serves as a valuable marker for studying late DE cell behavior.