Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Labeling DNA Probes03:31

Labeling DNA Probes

9.7K
DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...
9.7K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Mitochondrial asymmetry shifts T cell fate.

Nature cell biology·2026
Same author

Mitochondrial fusion and altered beta-oxidation drive muscle wasting in a Drosophila cachexia model.

EMBO reports·2024
Same author

Scribble and E-cadherin cooperate to control symmetric daughter cell positioning by multiple mechanisms.

Journal of cell science·2023
Same author

E-cadherin in developing murine T cells controls spindle alignment and progression through β-selection.

Science advances·2023
Same author

Stepwise progression of β-selection during T cell development involves histone deacetylation.

Life science alliance·2022
Same author

A Predictive Model for Super-Response to Cardiac Resynchronization Therapy: The QQ-LAE Score.

Cardiology research and practice·2020

Related Experiment Video

Updated: Mar 20, 2026

Highly Multiplexed, Super-resolution Imaging of T Cells Using madSTORM
08:43

Highly Multiplexed, Super-resolution Imaging of T Cells Using madSTORM

Published on: June 24, 2017

7.8K

Dense small molecule labeling enables activator-dependent STORM by proximity mapping.

Ye Chen1,2, Min Gu2,3, Peter W Gunning4

  • 1Immune Signalling Laboratory, Peter MacCallum Cancer Centre, East Melbourne, VIC, Australia.

Histochemistry and Cell Biology
|June 2, 2016
PubMed
Summary

Small molecule labels improve activator-dependent STORM imaging by increasing label density. This method enables multi-channel 3D imaging and provides insights into labeled structure conformation.

Keywords:
ActinMulti-channel imagingSingle-molecule localization microscopySmall molecule labelsSuper-resolution imaging

More Related Videos

Identification of Small Molecule-binding Proteins in a Native Cellular Environment by Live-cell Photoaffinity Labeling
10:49

Identification of Small Molecule-binding Proteins in a Native Cellular Environment by Live-cell Photoaffinity Labeling

Published on: September 20, 2016

13.4K
Super-Resolution Imaging of Bacterial Secreted Proteins Using Genetic Code Expansion
13:11

Super-Resolution Imaging of Bacterial Secreted Proteins Using Genetic Code Expansion

Published on: February 10, 2023

2.0K

Related Experiment Videos

Last Updated: Mar 20, 2026

Highly Multiplexed, Super-resolution Imaging of T Cells Using madSTORM
08:43

Highly Multiplexed, Super-resolution Imaging of T Cells Using madSTORM

Published on: June 24, 2017

7.8K
Identification of Small Molecule-binding Proteins in a Native Cellular Environment by Live-cell Photoaffinity Labeling
10:49

Identification of Small Molecule-binding Proteins in a Native Cellular Environment by Live-cell Photoaffinity Labeling

Published on: September 20, 2016

13.4K
Super-Resolution Imaging of Bacterial Secreted Proteins Using Genetic Code Expansion
13:11

Super-Resolution Imaging of Bacterial Secreted Proteins Using Genetic Code Expansion

Published on: February 10, 2023

2.0K

Area of Science:

  • Microscopy
  • Biophysics
  • Molecular Imaging

Background:

  • Stochastic optical reconstruction microscopy (STORM) offers high resolution but struggles with multi-channel 3D imaging due to chromatic aberrations and alignment issues.
  • Current activator-dependent STORM methods using single antibody-linked labels suffer from low labeling density and large probe size, limiting resolution.

Purpose of the Study:

  • To develop a small molecule labeling strategy for activator-dependent STORM to overcome limitations of antibody-based approaches.
  • To enhance labeling density and enable high-resolution multi-channel 3D STORM imaging.

Main Methods:

  • Investigated small molecule labels conjugated to phalloidin or mCling for activator-dependent STORM.
  • Explored linking reporters and activators to separate small molecules within 3.5 nm proximity.
  • Optimized activation based on activator choice, density, and laser parameters.

Main Results:

  • Demonstrated feasibility of small molecule labeling for activator-dependent STORM.
  • Achieved higher labeling density compared to antibody-based methods.
  • Showcased effective multi-channel 3D STORM imaging capabilities.

Conclusions:

  • Small molecule labeling is a viable strategy to enhance activator-dependent STORM for high-resolution 3D imaging.
  • The method improves resolution by increasing labeling density and offers insights into molecular proximity and structure conformation.