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Ussing Chamber Technique to Measure Intestinal Epithelial Permeability

Sadasivan Vidyasagar1, Gordon MacGregor2

  • 1Department of Radiation Oncology, University of Florida Shands Cancer Center, Cancer and Genetics Research Complex, 2033 Mowry Road, Box 103633, Gainesville, FL, 32610, USA. vidyasagar@ufl.edu.

Methods in Molecular Biology (Clifton, N.J.)
|June 2, 2016
PubMed
Summary

This study details methods for measuring epithelial barrier function using electrophysiological parameters and particle flux. Techniques include Ussing chamber analysis for transepithelial electrical resistance and nonionic particle permeability.

Keywords:
Diffusion potentialParacellular permeabilityTight junctionTransepithelial resistance

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