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Simultaneous Measurement of HDAC1 and HDAC6 Activity in HeLa Cells Using UHPLC-MS
Published on: August 10, 2017
Analysis of Expression and Functions of Histone Deacetylase 6 (HDAC6)
Miao Li1, Yan Zhuang2, Bin Shan3
1Department of Pathogen Biology, College of Basic Medical Sciences, China Medical University, Shenyang, Liaoning, People's Republic of China.
Abstract:
Histone deacetylase 6 (HDAC6) is a member of class IIb HDAC family. HDAC6 exists predominantly in the cytoplasm and deacetylates mainly non-histone proteins in the cytoplasm. Via its deacetylase and ubiquitin binding domains, HDAC6 regulates microtubules, cytoskeleton, intracellular trafficking, and cellular responses to stress. HDAC6 plays a central role in physiology and pathobiology in various organs and tissues. Herein we describe the methods for analysis of expression and function of HDAC6 in diverse physiological and pathological contexts.
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