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Updated: Mar 20, 2026
Deacetylation Assays to Unravel the Interplay between Sirtuins SIRT2 and Specific Protein-substrates
Published on: February 27, 2016
Cloning and Characterization of Sirtuin3 (SIRT3)
Andy W C Man1, Bo Bai1, Yu Wang2
1Department of Pharmacology and Pharmacy, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Level 2, Laboratory Block, 21 Sassoon Road, Pokfulam, Hong Kong, China.
Abstract:
Mitochondria play a pivotal role in maintaining cellular homeostasis and regulating longevity. SIRT3 is a mitochondrial sirtuin mediating the deacetylation of various metabolic and antioxidant enzymes, in turn controlling energy metabolism, stress resistance, and the pace of ageing. To study the function of SIRT3, a proteomics-based approach is employed for identifying the protein-binding partners of this enzyme in mitochondria.
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