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Selective Labelling of Cell-surface Proteins using CyDye DIGE Fluor Minimal Dyes
Published on: November 26, 2008
15.7K
High performance CCD camera system for digitalisation of 2D DIGE gels.
Annemieke Strijkstra1,2, Kathleen Trautwein1,2, Stefan Roesler3
1General and Molecular Microbiology, Institute for Chemistry and Biology of the Marine Environment (ICBM), Carl von Ossietzky University Oldenburg, Oldenburg, Germany.
Proteomics
|June 3, 2016
Summary
A new camera system offers accurate 2D DIGE gel digitalization, comparable to laser scanners. This advancement provides a viable alternative for analyzing differential protein expression in environmental bacteria.
Area of Science:
- Proteomics
- Biotechnology
- Environmental Microbiology
Background:
- Accurate digitalization of 2D DIGE gels is crucial for differential proteome analysis.
- Advancements in charge-coupled device (CCD) camera systems offer potential for gel imaging.
Purpose of the Study:
- To evaluate a CCD camera system (Intas Advanced 2D Imager) as an alternative to a laser scanner (Typhoon 9400).
- To assess the performance of CCD-based digitalization for 2D DIGE gels using environmental bacteria.
Main Methods:
- Digitalization of 2D DIGE gels from three environmental bacteria using both CCD camera and laser scanner systems.
- Comparative analysis of protein abundance changes, spot position, volume, linear range, and limit of detection.
Main Results:
- The CCD camera system demonstrated performance comparable to the high-end laser scanner.
- Similar protein abundance changes were observed irrespective of spot position and volume.
- The linear range and limit of detection were consistent between the two systems.
Conclusions:
- The Intas Advanced 2D Imager provides a viable and comparable alternative for 2D DIGE gel digitalization.
- This CCD camera system supports sensitive and accurate analysis of differential protein profiles.
