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In vitro Mutagenesis

In vitro Mutagenesis

In-vitro Mutagenesis

In-vitro Mutagenesis

To learn more about the function of a gene, researchers can observe what happens when the gene is inactivated or “knocked out,” by creating genetically engineered knockout animals. Knockout mice have been particularly useful as models for human diseases such as cancer, Parkinson’s disease, and diabetes.

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Related Experiment Video

Updated: Mar 20, 2026

A Protocol for Functional Assessment of Whole-Protein Saturation Mutagenesis Libraries Utilizing High-Throughput Sequencing

A Protocol for Functional Assessment of Whole-Protein Saturation Mutagenesis Libraries Utilizing High-Throughput Sequencing

Published on: July 3, 2016

The Parasol Protocol for computational mutagenesis.

P G A Aronica¹, C Verma², B Popovic³

¹Department of Chemistry and Institute of Chemical Biology, Imperial College London, South Kensington Campus, Exhibition Road, London SW7 2AZ, UK.

Protein Engineering, Design & Selection : PEDS

|June 4, 2016

Summary

This summary is machine-generated.

We developed the Parasol Protocol for computational site-directed mutagenesis to accelerate therapeutic peptide and protein discovery. This automated method shows good agreement with experimental data for stapled peptides.

Keywords:

in silico mutation molecular dynamics

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Last Updated: Mar 20, 2026

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Published on: July 3, 2016

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Published on: November 14, 2016

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Published on: May 15, 2018

Area of Science:

Biochemistry and Molecular Biology
Computational Chemistry
Drug Discovery

Background:

Peptides and proteins are crucial therapeutic agents.
Predictive modeling aids in their discovery and development.
Computational methods can optimize research workflows.

Purpose of the Study:

To introduce the Parasol Protocol, a novel computational tool.
To enable efficient, automated site-directed mutagenesis.
To validate the protocol's accuracy in predicting experimental outcomes.

Main Methods:

Developed the Parasol Protocol using the AMBER Molecular Dynamics (MD) package.
Implemented a dynamic method for computational site-directed mutagenesis.
Automated the mutation process between any amino acid pairs.

Main Results:

The Parasol Protocol facilitates computationally expedient mutagenesis.
Demonstrated the protocol's utility with a stapled peptide case study.
Achieved good agreement between computational predictions and experimental results.

Conclusions:

The Parasol Protocol is a valuable tool for therapeutic peptide and protein research.
This method can significantly direct and accelerate drug discovery workflows.
Computational mutagenesis offers a powerful approach to experimental validation.