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Lipid Bilayer Vesicle Generation Using Microfluidic Jetting
Published on: February 21, 2014
Repetitive formation of optically-observable planar lipid bilayers by rotating chambers on a microaperture
Fumiaki Tomoike1, Taishi Tonooka, Toshihisa Osaki
1Center for International Research on Integrative Biomedical Systems (CIBiS), Institute of Industrial Science (IIS), The University of Tokyo, 4-6-1 Komaba Meguro-ku, Tokyo 153-8505, Japan. takeuchi@iis.u-tokyo.ac.jp.
Abstract:
Optical observation of a planar lipid bilayer is an effective method of lipid bilayer characterization. However, previous methods for optically observable lipid bilayer formation are unsuitable for repetitive formation of lipid bilayers. In this paper, we propose a system that facilitates repetitive formation of horizontal lipid bilayers via mechanical rotation of the rotating part. We show that multiple bilayers can be observed within a short period, and that the electrical and optical characteristics of a bilayer can be analyzed simultaneously.

