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Photoconversion of Purified Fluorescent Proteins and Dual-probe Optical Highlighting in Live Cells
Published on: June 26, 2010
A highly selective and sensitive photoinduced electron transfer (PET) based HOCl fluorescent probe in water and its
Lijuan Liang1, Chang Liu, Xiaojie Jiao
1School of Materials Science and Engineering, Harbin Institute of Technology, Harbin 150001, China.
Abstract:
A probe based on the phenothiazine-acridine orange conjugate (Ptz-AO) has been designed and synthesized for the sensitive and selective detection of HOCl. Ptz-AO has excellent properties, including pH-independence of fluorescence, high resistance to photobleaching, and response in real time. The value of Ptz-AO was confirmed by exogenous, endogenous and the real-time imaging of HOCl in vitro using a fluorescence microscope.
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