Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Ribosome Profiling

Ribosome Profiling

Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Quo vadis, BGA? A collaborative EDNAP exercise on the challenges and progress in forensic biogeographical ancestry inference.

Forensic science international. Genetics·2026

Same author

Assessing the impact of bone type on the accuracy of methylation-based forensic age estimation.

Forensic science international. Genetics·2026

Same author

From unsupervised selection of AIMs to likelihood-based BGA inference: Challenges revealed by OpenADMIXTURE and GENOGEOGRAPHER.

Forensic science international. Genetics·2026

Same author

Challenges in mRNA-based time since deposition estimation: A multiplex MPS primer panel investigation.

Forensic science international. Genetics·2026

Same author

Methylation-based forensic age estimation on different biogeographic backgrounds: A study on Central Europeans, East Asians and West Africans.

Forensic science international. Genetics·2025

Same author

The influence of cancer on a forensic age estimation tool.

Aging·2025

Search research articles

Related Experiment Video

Updated: Mar 20, 2026

Enhanced Genetic Analysis of Single Human Bioparticles Recovered by Simplified Micromanipulation from Forensic ‘Touch DNA’ Evidence

Enhanced Genetic Analysis of Single Human Bioparticles Recovered by Simplified Micromanipulation from Forensic ‘Touch DNA’ Evidence

Published on: March 9, 2015

Body Fluid Identification Using mRNA Profiling.

Amy D Roeder¹, Cordula Haas²

¹Cellmark, Blacklands Way, Abingdon, Oxfordshire, OX14 1DY, UK. aroeder@cellmark.co.uk.

Methods in Molecular Biology (Clifton, N.J.)

|June 5, 2016

Summary

This summary is machine-generated.

RNA analysis identifies forensically relevant body fluids like saliva and blood using specific mRNA and bacterial RNA markers. This method allows for multiplex PCR, maximizing analyses from limited forensic samples.

Keywords:

Body fluid identification Forensic mRNA profiling

More Related Videos

Cerebrospinal Fluid MicroRNA Profiling Using Quantitative Real Time PCR

Cerebrospinal Fluid MicroRNA Profiling Using Quantitative Real Time PCR

Published on: January 22, 2014

Method for the Isolation and Identification of mRNAs, microRNAs and Protein Components of Ribonucleoprotein Complexes from Cell Extracts using RIP-Chip

Method for the Isolation and Identification of mRNAs, microRNAs and Protein Components of Ribonucleoprotein Complexes from Cell Extracts using RIP-Chip

Published on: September 29, 2012

Related Experiment Videos

Last Updated: Mar 20, 2026

Enhanced Genetic Analysis of Single Human Bioparticles Recovered by Simplified Micromanipulation from Forensic ‘Touch DNA’ Evidence

Enhanced Genetic Analysis of Single Human Bioparticles Recovered by Simplified Micromanipulation from Forensic ‘Touch DNA’ Evidence

Published on: March 9, 2015

Cerebrospinal Fluid MicroRNA Profiling Using Quantitative Real Time PCR

Cerebrospinal Fluid MicroRNA Profiling Using Quantitative Real Time PCR

Published on: January 22, 2014

Method for the Isolation and Identification of mRNAs, microRNAs and Protein Components of Ribonucleoprotein Complexes from Cell Extracts using RIP-Chip

Method for the Isolation and Identification of mRNAs, microRNAs and Protein Components of Ribonucleoprotein Complexes from Cell Extracts using RIP-Chip

Published on: September 29, 2012

Area of Science:

Forensic science
Molecular biology
Genetics

Background:

RNA analysis is crucial for identifying human body fluids in forensic investigations.
Specific human mRNA and bacterial RNA markers exist for various body fluids.

Purpose of the Study:

To highlight the utility of RNA analysis for forensically relevant body fluid identification.
To emphasize the efficiency of multiplexing RNA markers for comprehensive analysis.

Main Methods:

Identification of multiple human mRNA and bacterial RNA markers for saliva, blood, menstrual blood, cervicovaginal fluid, and semen.
Coextraction of RNA and DNA from biological samples.
Multiplex PCR amplification of various RNA markers in a single reaction.

Main Results:

Established RNA markers for differentiating key body fluids.
Demonstrated successful coextraction of RNA and DNA.
Validated multiplex PCR for simultaneous detection of multiple RNA markers.

Conclusions:

RNA analysis provides a robust method for body fluid identification in forensic contexts.
Multiplexing RNA markers enhances analytical efficiency, conserving precious forensic samples.