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Related Experiment Video

Updated: Mar 20, 2026

Isolating LacZ-expressing Cells from Mouse Inner Ear Tissues using Flow Cytometry

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Published on: December 23, 2011

Profiling Specific Inner Ear Cell Types Using Cell Sorting Techniques.

Taha A Jan¹, Lina Jansson², Patrick J Atkinson²

¹Department of Otolaryngology, Massachusetts Eye and Ear Infirmary, Harvard Medical School, Boston, MA, 02114, USA.

Methods in Molecular Biology (Clifton, N.J.)

|June 5, 2016

Summary

This summary is machine-generated.

Researchers developed new methods to isolate specific inner ear cells from neonatal mouse cochlea. This technique uses fluorescence-activated cell sorting for detailed cell biology and gene expression studies.

Keywords:

Fluorescence-activated cell sorting Inner ear Profiling Single cell analysis

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A Combinatorial Single-cell Approach to Characterize the Molecular and Immunophenotypic Heterogeneity of Human Stem and Progenitor Populations

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Published on: October 25, 2018

Area of Science:

Cell Biology
Genomics
Neuroscience

Background:

Investigating specific cell types is crucial for understanding cell biology and gene expression.
Isolating individual cell populations aids in detailed analysis and characterization.
Studying inner ear cells is challenging due to limited cell numbers and markers.

Purpose of the Study:

To describe methods for selective labeling and isolation of inner ear cell types.
To enable detailed analysis of cell biology and gene expression in the inner ear.
To overcome challenges associated with inner ear cell isolation.

Main Methods:

Utilized fluorescence-activated cell sorting (FACS).
Developed selective labeling techniques for inner ear cells.
Applied methods to neonatal mouse cochlea samples.

Main Results:

Successfully isolated distinct inner ear cell populations.
Demonstrated the feasibility of FACS for inner ear cell research.
Provided a basis for further characterization of cell-specific functions.

Conclusions:

Selective cell isolation using FACS is effective for inner ear research.
These methods facilitate the study of cell biology and gene expression in specific inner ear cell types.
The described techniques address the challenges of limited cell numbers and markers in the inner ear.