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Related Concept Videos

Genetic Screens02:46

Genetic Screens

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Genetic screens are tools used to identify genes and mutations responsible for phenotypes of interest. Genetic screens help identify individuals or a group of people at risk of developing  genetic diseases and help them with early intervention, targeted therapy, and reproductive options.
Forward genetic screens
Forward or “classical” genetic screens involve creating random mutations in an organism’s DNA using radiation, mutagens, or insertion of additional bases, which...
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CRISPR/Cas9 Genome Editing01:28

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The CRISPR-Cas system serves as a bacterial defense mechanism against invading genetic elements such as viruses and plasmids, forming the foundation for its adaptation as a powerful genome-editing tool. Originally discovered in prokaryotes, this system has been repurposed to revolutionize genetic engineering across a wide range of organisms, including plants, animals, and humans. The core component, Cas9, is an endonuclease derived from Streptococcus pyogenes, capable of introducing...
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CRISPR01:59

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Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced...
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Related Experiment Video

Updated: Mar 20, 2026

Pooled CRISPR-Based Genetic Screens in Mammalian Cells
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Pooled CRISPR-Based Genetic Screens in Mammalian Cells

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Genome-scale CRISPR pooled screens.

Neville E Sanjana1

  • 1New York Genome Center, New York, NY 10013, USA; Center for Genomics and Systems Biology, Department of Biology, New York University, New York, NY 10012, USA.

Analytical Biochemistry
|June 5, 2016
PubMed
Summary

Pooled CRISPR screens enable large-scale genetic analysis by easily programming DNA manipulation. This review covers fundamental concepts, methods, and recent biological results from loss-of-function and gain-of-function screens.

Keywords:
CRISPRCancerCas9Genetic screensGenome engineeringNoncoding

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Area of Science:

  • Molecular Biology
  • Genetics
  • Bioengineering

Background:

  • Genome editing technologies, particularly clustered regularly interspaced short palindromic repeats (CRISPR) systems, have revolutionized targeted DNA manipulation.
  • The programmability of CRISPR via short oligonucleotides facilitates the rapid creation of large-scale libraries essential for functional genetic screens.

Purpose of the Study:

  • To present fundamental concepts and methodologies for pooled CRISPR screens.
  • To review key biological findings from recent genome-scale loss-of-function and gain-of-function screens.
  • To explore emerging trends in pooled screening, including novel effector domains and noncoding genome applications.

Main Methods:

  • Utilizing CRISPR-Cas systems for targeted gene editing.
  • Designing and synthesizing large-scale oligonucleotide libraries for pooled screens.
  • Analyzing genome-scale loss-of-function and gain-of-function screening data.

Main Results:

  • Demonstrated the utility of pooled CRISPR screens for large-scale genetic analysis.
  • Summarized significant biological insights derived from recent genome-scale screens.
  • Highlighted the potential of CRISPR technology in functional genomics and noncoding genome research.

Conclusions:

  • Pooled CRISPR screens are powerful tools for advancing functional genomics.
  • Future directions include developing novel effector domains and expanding applications to the noncoding genome.
  • CRISPR technology continues to drive innovation in genetic research and biological discovery.