Fourier analysis of the nerve fiber layer by digital imaging techniques

We introduce a new method to examine the retinal nerve fiber layer (RNFL) that may detect early changes in its structure due to glaucoma. Viewed in the conventional manner, in a plane perpendicular to the retina, we get an estimation of the structural integrity of the RNFL. Viewed from a plane parallel to the retina, the RNFL forms a contour surface of hills and valleys. In our method red free fundus slides are digitally acquired. A waveform, representing a topographic profile cut through the resulting representation of the RNFL contour surface, was analyzed using Fourier's transform. Three cycles/mm in the Fourier power spectrum was used as an arbitrary cutoff to discriminate normal from glaucomatous RNFL. The Fourier power spectrum from normal RNFL was shifted to higher spatial frequencies, while the power spectrum from glaucomatous RNFL was shifted to lower spatial frequencies. Thus, Fourier Analysis can reveal information about such a wave form which is not obvious by visual inspection or by densitometric techniques. This method may allow for an even earlier diagnosis of injury to the optic nerve due to glaucoma.