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Updated: Mar 20, 2026

Multilayer Mounting for Long-term Light Sheet Microscopy of Zebrafish

Multilayer Mounting for Long-term Light Sheet Microscopy of Zebrafish

Published on: February 27, 2014

Second harmonic generation microscopy in zebrafish.

D C LeBert¹, J M Squirrell¹, A Huttenlocher¹

¹University of Wisconsin-Madison, Madison, WI, United States.

Methods in Cell Biology

|June 7, 2016

Summary

This summary is machine-generated.

Second harmonic generation (SHG) microscopy visualizes leukocyte-collagen interactions during zebrafish wound healing. This optical imaging technique monitors intrinsic contrast for studying inflammation and tissue repair processes.

Keywords:

Macrophage Multiphoton SHG Wound healing Zebrafish

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Area of Science:

Biomedical Optics
Cellular Imaging
Zebrafish Models

Background:

Optical imaging advances enable noninvasive study of cellular processes.
Second harmonic generation (SHG) microscopy offers intrinsic contrast imaging, particularly for collagen.
SHG combined with multiphoton fluorescence microscopy visualizes cell-extracellular environment interactions.

Purpose of the Study:

To present practical aspects of using SHG microscopy.
To image leukocyte-collagen interactions during zebrafish wound healing.
To highlight SHG's utility in studying inflammation and tissue repair.

Main Methods:

Utilizing second harmonic generation (SHG) microscopy.
Coupling SHG with multiphoton fluorescence microscopy.
Employing zebrafish as a model organism for wound healing studies.

Main Results:

Demonstrated SHG's capability to image leukocyte-collagen dynamics.
Provided insights into cellular interactions within the wound microenvironment.
Showcased the practical application of SHG in live and fixed samples.

Conclusions:

SHG microscopy is a valuable tool for investigating leukocyte-collagen interactions in wound healing.
Zebrafish models are suitable for studying inflammatory and repair processes using advanced optical techniques.
This approach enhances understanding of the extracellular matrix's role in tissue regeneration.