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Updated: Mar 20, 2026

Isolation and Identification of Bacterial Strains from Skin of Terrestrial Amphibians
Published on: June 17, 2025
Emticicia paludis sp. nov., isolated from wetland freshwater
Heeyoung Kang1, Haneul Kim1, Yochan Joung2
1Department of Bioscience and Biotechnology, Hankuk University of Foreign Studies, Gyeonggi 17035, Republic of Korea.
Abstract:
A novel bacterium, designated HMF3850T, was isolated from freshwater collected from the Gyeong-an wetland, Republic of Korea. Cells were Gram-stain-negative, aerobic, straight rods, non-motile, and oxidase- and catalase-positive. Growth was observed at pH 6.0-8.0 and at 10-30 °C on R2A agar. Comparative analysis of 16S rRNA gene sequences revealed that strain HMF3850T is a member of the genus Emticicia, sharing highest sequence similarities with Emticicia aquatica HMF2925T (96.7 % 16S rRNA gene sequence similarity), Emticicia sediminis JBR12T (95.0 %), Emticicia ginsengisoliGsoil 085T (94.9 %) and Emticicia oligotrophica DSM 17448T (94.7 %). The major fatty acids were iso-C15 : 0, summed feature 3 (C16 : 1ω7c and/or C16 : 1ω6c) and C16 : 0. The predominant respiratory quinone was MK-7. The major polar lipids were phosphatidylethanolamine, phosphatidylinositol, one unidentified aminophospholipid, two unidentified aminolipids and eight unidentified polar lipids. The DNA G+C content was 38.5 mol %. On the basis of the evidence presented in this study, strain HMF3850T represents a novel species of the genus Emticicia, for which the name Emticicia paludis sp. nov. is proposed. The type strain is HMF3850T (=KCTC 42851T=CECT 9087T).
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