Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Atomic Force Microscopy01:08

Atomic Force Microscopy

4.7K
Atomic force microscopy (AFM) is a type of scanning probe microscopy that can analyze topographic details of various specimens like ceramics, glass, polymers, and biological samples. AFM offers over 1000 times more resolution than the optical imaging system. Images generated from AFM are three-dimensional surface profiles, offering an advantage over the flat, two-dimensional images from other imaging techniques.
The AFM Probe
The probe is regarded as the heart of any AFM setup and comprises the...
4.7K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

AI-enhanced cardiac digital twins extend drug proarrhythmic risk assessment through experimental data uncertainty propagation and overdose exploration: A loperamide case study.

Regulatory toxicology and pharmacology : RTP·2026
Same author

Comparing capillary coatings for protein separation by capillary electrophoresis.

Analytica chimica acta·2026
Same author

VRSPi: towards a neuroadaptive VR exposure therapy system for spider phobia.

Frontiers in human neuroscience·2026
Same author

Determination of the concentration and size-distribution of residual capsular poly(N-acetyl neuraminic acid) from outer membrane vesicle vaccines by micellar electrokinetic capillary chromatography.

Analytica chimica acta·2026
Same author

Critical parameters for achieving high efficiency and reproducible double-chained cationic surfactant coatings for protein separation by capillary electrophoresis.

Journal of chromatography. A·2026
Same author

Protein Amyloid and Synthetic Polymer Self-Assemblies: Similar Challenges for Their Preparation Associated with Shared Properties.

Biomacromolecules·2026

Related Experiment Video

Updated: Mar 20, 2026

Investigating Single Molecule Adhesion by Atomic Force Spectroscopy
09:48

Investigating Single Molecule Adhesion by Atomic Force Spectroscopy

Published on: February 27, 2015

10.9K

Mapping molecular adhesion sites inside SMIL coated capillaries using atomic force microscopy recognition imaging.

Michael Leitner1, Lorenz G Stock2, Lukas Traxler1

  • 1Institute of Biophysics, Johannes Kepler University Linz, Gruberstrasse 40, 4020 Linz, Austria.

Analytica Chimica Acta
|June 7, 2016
PubMed
Summary

This study optimized topography and recognition imaging (TREC) to characterize capillary zone electrophoresis (CZE) coatings. TREC successfully mapped the nanoscale charge distribution of Successive Multiple Ionic Layer (SMIL) coatings, demonstrating its functional investigation capabilities.

Keywords:
Adhesion forcesAtomic force microscopyCapillary electrophoresisRecognition imagingSMIL coating

More Related Videos

Functionalization of Atomic Force Microscope Cantilevers with Single-T Cells or Single-Particle for Immunological Single-Cell Force Spectroscopy
10:06

Functionalization of Atomic Force Microscope Cantilevers with Single-T Cells or Single-Particle for Immunological Single-Cell Force Spectroscopy

Published on: July 10, 2019

8.0K
Covalent Attachment of Single Molecules for AFM-based Force Spectroscopy
10:37

Covalent Attachment of Single Molecules for AFM-based Force Spectroscopy

Published on: March 16, 2020

10.4K

Related Experiment Videos

Last Updated: Mar 20, 2026

Investigating Single Molecule Adhesion by Atomic Force Spectroscopy
09:48

Investigating Single Molecule Adhesion by Atomic Force Spectroscopy

Published on: February 27, 2015

10.9K
Functionalization of Atomic Force Microscope Cantilevers with Single-T Cells or Single-Particle for Immunological Single-Cell Force Spectroscopy
10:06

Functionalization of Atomic Force Microscope Cantilevers with Single-T Cells or Single-Particle for Immunological Single-Cell Force Spectroscopy

Published on: July 10, 2019

8.0K
Covalent Attachment of Single Molecules for AFM-based Force Spectroscopy
10:37

Covalent Attachment of Single Molecules for AFM-based Force Spectroscopy

Published on: March 16, 2020

10.4K

Area of Science:

  • Analytical Chemistry
  • Surface Science
  • Nanotechnology

Background:

  • Capillary zone electrophoresis (CZE) is vital for separating diverse analytes, but its resolution depends heavily on capillary inner surface coatings.
  • Developing stable coatings with switchable surface charges, such as Successive Multiple Ionic Polymer (SMIL) layers, is crucial for optimal CZE performance.
  • Characterizing these complex SMIL coatings at the nanoscale remains a significant technical challenge.

Purpose of the Study:

  • To optimize and apply topography and recognition imaging (TREC) for detailed nanoscale characterization of SMIL coatings in electrophoresis capillaries.
  • To investigate the charge distribution and homogeneity of differently composed and treated SMIL coatings.
  • To demonstrate the efficacy of TREC as a functional investigation technique for nanoscale surface analysis.

Main Methods:

  • Atomic force microscopy (AFM) was adapted for nanoscale topographical and adhesion mapping.
  • The AFM scanning tip was functionalized with avidin molecules to act as a single-molecule biosensor for detecting negatively charged regions.
  • Topography and recognition imaging (TREC) was employed to simultaneously acquire surface topography and adhesion maps of SMIL-coated capillaries.

Main Results:

  • TREC successfully generated high-resolution adhesion maps of SMIL coatings, revealing nanoscale charge distribution.
  • The study compared various SMIL coating compositions and treatments based on their charge characteristics.
  • The capability of TREC to provide functional nanoscale insights into electrophoresis capillary coatings was validated.

Conclusions:

  • Topography and recognition imaging (TREC) is a powerful technique for the nanoscale functional characterization of electrophoresis capillary coatings.
  • TREC provides valuable information on surface charge distribution and homogeneity, crucial for optimizing CZE separation performance.
  • This method advances the understanding and development of advanced capillary coatings for high-resolution electrophoresis.