Related Experiment Video
Updated: Mar 19, 2026
09:44
Oral Biofilm Analysis of Palatal Expanders by Fluorescence In-Situ Hybridization and Confocal Laser Scanning Microscopy
Published on: October 20, 2011
16.7K
Microbial complexes levels in conventional and self-ligating brackets.
Ana Zilda Nazar Bergamo1, Paulo Nelson-Filho2, Marcela Cristina Damião Andrucioli2
1Department of Pediatric Clinic, School of Dentistry of Ribeirão Preto, University of São Paulo, Avenida do Café, S/N, Monte Alegre, Ribeirão Preto, SP, CEP: 14040-904, Brazil. anaznbergamo@gmail.com.
Clinical Oral Investigations
|June 9, 2016
Summary
Orthodontic bracket design impacts oral bacteria levels, potentially increasing periodontal disease risk. Further research is needed to develop preventive strategies for orthodontic patients.
Area of Science:
- Oral microbiology
- Periodontal disease research
- Orthodontic material science
Background:
- Orthodontic treatment involves bracket placement, which can alter the oral environment.
- Understanding the relationship between bracket design and bacterial colonization is crucial for preventing periodontal disease.
Purpose of the Study:
- To evaluate bacterial levels in saliva and in situ.
- To determine if bracket design influences the risk of periodontal disease.
Main Methods:
- Twenty patients received self-ligating and conventional brackets.
- Saliva and bracket samples were analyzed using checkerboard DNA-DNA hybridization.
- Data was statistically evaluated using non-parametric tests.
Main Results:
- A significant increase in 15 of 22 bacterial species was observed in saliva.
- Self-ligating brackets showed higher incidence of orange and red complex bacteria.
- Campylobacter rectus levels differed significantly among bracket types, with In-Ovation®R showing the highest values.
Conclusions:
- Bracket design appears to influence bacterial species linked to periodontal disease.
- Different bracket types affect bacterial adherence.
- Consideration of bracket design is recommended for orthodontic treatment planning to mitigate periodontal risks.
Related Concept Videos
01:27The Oral Microbiota
7
The oral microbiome includes a complex ecosystem comprising over 700 microbial species, identified through genomic sequencing and culture-based analyses to date. This community includes a core microbiome, found universally among individuals, and a variable component influenced by environmental factors such as diet, lifestyle, and host genetics. Site-specific conditions, including oxygen gradients, pH levels, and nutrient availability, determine the spatial distribution of these microorganisms...
7
01:28Development of the Oral Microbiota
7
The establishment of the oral microbiome begins before birth, challenging the long-held belief that the fetal oral cavity is sterile. The presence of oral microbes such as Streptococcus and Fusobacterium in amniotic fluid suggests that microbial exposure may occur in utero, potentially through translocation from the maternal oral or gastrointestinal tract. This early colonization primes the neonatal immune system and sets the stage for subsequent microbial succession. Maternal health,...
7
01:29Biofilms
1.8K
Biofilms are complex communities of microorganisms encased in a self-produced extracellular polysaccharide matrix attached to surfaces. These microbial consortia can include single or multiple species, providing enhanced survival benefits by forming organized, multilayered structures.The formation of biofilms occurs through four key stages: attachment, colonization, development, and dispersal.During attachment, free-swimming planktonic cells adhere to a surface, often facilitated by...
1.8K
01:29Microbiota of the Respiratory Tract
1
The human respiratory tract, comprising the upper and lower segments, serves as a critical interface with the external environment. The upper respiratory tract (URT)—including the nostrils, sinuses, pharynx, and oropharynx—is heavily colonized by microbes, while the lower respiratory tract (LRT), composed of the larynx, trachea, bronchi, and lungs, was long thought to be sterile. However, recent molecular studies have revealed that the lungs are not devoid of microbes but act more...
1