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Related Concept Videos

Ribosome Profiling02:24

Ribosome Profiling

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Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
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General Transcription Factors01:30

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Tissue-specific transcription factors contribute to diverse cellular functions in mammals. For example, the gene for beta globin, a major component of hemoglobin, is present in all cells of the body. However, it is only expressed in red blood cells because the transcription factors that can bind to the promoter sequences of the beta globin gene are only expressed in these cells. Tissue-specific transcription factors also ensure that mutations in these factors may impair only the function of...
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The gene expression in cells is regulated at different stages: (i) transcription, (ii) RNA processing, (iii) RNA localization, and (iv) translation. Transcriptional regulation is mediated by regulatory proteins such as transcription factors, activators, or repressors—these control gene expression by initiating or inhibiting the transcription of genes. Once a precursor or pre-mRNA is produced, it undergoes post-transcriptional modification, including 5' capping, splicing, and the...
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A gene is a stretch of DNA that serves as the blueprint for functional RNAs and proteins. Since DNA is comprised  of nucleotides and proteins are comprised of amino acids, a mediator is required to convert the information encoded in DNA into proteins. This mediator is the messenger RNA (mRNA). mRNA copies the blueprint from DNA by a process called transcription. In eukaryotes, transcription occurs in the nucleus by complementary base-pairing with the DNA template. The mRNA is then...
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Transcription01:17

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Transcription is the synthesis of RNA from a DNA sequence by RNA polymerase. It is the first step in producing a protein from a gene sequence. Additionally, many other proteins and regulatory sequences are involved in correctly synthesizing messenger RNA (mRNA). Transcriptional regulation is responsible for the differentiation of different types of cells and often for the proper cellular response to environmental signals.
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Multicellular organisms contain a variety of structurally and functionally distinct cell types, but the DNA in all the cells originated from the same parent cells. The differences in the cells can be attributed to the differential gene expression. Liver cells, whose functions include detoxification of blood, production of bile to metabolize fats, and synthesis of proteins essential for metabolism, must express a specific set of genes to perform their functions. Gene expression also varies with...
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Related Experiment Video

Updated: Mar 19, 2026

Identification of Key Factors Regulating Self-renewal and Differentiation in EML Hematopoietic Precursor Cells by RNA-sequencing Analysis
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Global transcriptome analysis for identification of interactions between coding and noncoding RNAs during human

Nan Ding1,2, Jiafei Xi3,4, Yanming Li1

  • 1CAS Key Laboratory of Genome Sciences and Information, Beijing Institute of Genomics, Chinese Academy of Sciences, Beijing, 100101, China.

Frontiers of Medicine
|June 9, 2016
PubMed
Summary

This study integrates coding genes, microRNAs (miRNAs), and long noncoding RNAs (lncRNAs) during red blood cell development. It reveals lncRNAs as potential markers and uncovers noncoding RNA roles in regulating cell maturation and heme metabolism.

Keywords:
RNA-seqerythroid differentiationhematopoietic stem celllncRNAmiRNA

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Lentiviral-mediated Knockdown During Ex Vivo Erythropoiesis of Human Hematopoietic Stem Cells
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Lentiviral-mediated Knockdown During Ex Vivo Erythropoiesis of Human Hematopoietic Stem Cells
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Area of Science:

  • Transcriptomics
  • Molecular Biology
  • Hematopoiesis

Background:

  • Previous studies focused on individual RNA types during erythroid development.
  • A comprehensive analysis integrating coding genes, miRNAs, and lncRNAs is lacking.

Purpose of the Study:

  • To compare expression dynamics of coding genes, miRNAs, and lncRNAs during erythroid differentiation.
  • To explore transcriptome-level mechanisms controlling erythropoiesis.
  • To identify potential regulatory roles of noncoding RNAs in red blood cell development.

Main Methods:

  • High-throughput sequencing of transcriptome data.
  • Analysis of cord blood hematopoietic stem cells and erythroid differentiation stages.
  • Clustering analysis of differentially expressed genes.
  • Integrated analysis of coding and noncoding RNA interactions.

Main Results:

  • Long noncoding RNAs (lncRNAs) show promise as cell markers for erythroid differentiation.
  • Four subtypes of differentially expressed genes reflect dynamic changes in stemness, mid-differentiation, and maturation.
  • Noncoding RNAs are implicated in controlling blood cell maturation, heme metabolism, and responses to oxidative stress and DNA damage.

Conclusions:

  • This study provides a comprehensive resource for understanding normal erythropoiesis.
  • Integrated RNA analysis reveals novel insights into regulatory networks governing red blood cell development.
  • Findings facilitate further investigation of erythroid development and related disorders.