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Three-dimensional visualisation of soft biological structures by X-ray computed micro-tomography.

Tom Shearer1, Robert S Bradley2, L Araida Hidalgo-Bastida3

  • 1School of Materials, University of Manchester, Manchester M13 9PL, UK School of Mathematics, University of Manchester, Manchester M13 9PL, UK.

Journal of Cell Science
|June 10, 2016
PubMed
Summary

X-ray computed micro-tomography (microCT) offers a rapid alternative to traditional 3D imaging techniques for biological samples. This method provides sub-micron resolution for static and dynamic structural analysis, advancing biological research.

Keywords:
ArteriesMicroCTTendonTissue engineeringTomographyX-ray

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Area of Science:

  • Biophysics
  • Biomaterials Science
  • Microscopy

Background:

  • Traditional 3D imaging of biological samples is often time-consuming and specialized.
  • Current methods include serial sectioning and electron tomography, which have limitations.

Purpose of the Study:

  • To discuss the history and capabilities of X-ray computed micro-tomography (microCT) as an alternative 3D imaging technique.
  • To illustrate microCT's utility through diverse biological case studies.

Main Methods:

  • X-ray computed micro-tomography (microCT) for rapid 3D imaging.
  • Visualization of intact samples and dynamic changes at microsecond scales.
  • Case studies involving unstained rat arteries, stained collagen fascicles, chrysalis development, and cell distribution in tissue constructs.

Main Results:

  • MicroCT enables rapid imaging of internal 3D structures at sub-micron resolution.
  • Dynamic changes in living tissues can be visualized at microsecond timescales.
  • Demonstrated ability to visualize and quantify structural changes and cell distribution in various biological samples.

Conclusions:

  • MicroCT presents a powerful and versatile tool for 3D biological imaging.
  • Future advancements in detector technology and synchrotron sources promise enhanced real-time imaging capabilities.
  • MicroCT is poised to become a more routine technique in biological research.