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Published on: January 15, 2018
Glyco-gold nanoparticle shapes enhance carbohydrate-protein interactions in mammalian cells
Sivakoti Sangabathuni1, Raghavendra Vasudeva Murthy, Preeti Madhukar Chaudhary
1Indian Institute of Science Education and Research, Pashan, Pune 411008, India. rkikkeri@iiserpune.ac.in.
Abstract:
Advances in shape-dependent nanoparticle (NP) research have prompted a close scrutiny of the behaviour of nanostructures in vitro and in vivo. Data pertaining to cellular uptake and site specific sequestration of different shapes of NPs will undoubtedly assist researchers to design better nano-probes for therapeutic and imaging purposes. Herein, we investigated the shape dependent uptake of glyco-gold nanoparticles (G-AuNPs) in different cancer cell lines. Specifically, we have compared the behaviour of spherical, rod and star AuNPs with mannose and galactose conjugations. In vitro experiments showed that the rod-AuNPs exhibited the highest uptake over that of the star and spherical counterparts. Further, an investigation of the mechanism of the uptake clearly demonstrated clathrin mediated endocytosis of the specific G-AuNPs. These results reveal the benefits of different G-AuNP shapes in carbohydrate-mediated interactions.
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