Microarray-based long oligonucleotides probe designed for Brucella Spp. detection and identification of antibiotic...

Area of Science:

• Microbiology
• Molecular Biology
• Infectious Diseases

Background:

• Brucellosis, a zoonotic infection caused by Brucella spp., poses a significant global public health challenge.
• Current diagnostic methods, such as disk diffusion, cannot rapidly determine antibiotic resistance.
• Molecular techniques are essential for accurate Brucella detection and antibiotic resistance profiling.

Purpose of the Study:

• To design a microarray-based molecular diagnostic tool for Brucella spp.
• To simultaneously identify antibiotic resistance genes in Brucella.
• To develop a comprehensive diagnostic and genetic profiling approach for Brucellosis.

Main Methods:

• Design and synthesis of 16 solid-phase primers targeting antibiotic resistance genes.
• Design of 16 long oligonucleotide probes for specific gene identification.
• Development of a microarray assay for simultaneous Brucella detection and resistance gene profiling.

Main Results:

• The designed primers and probes can identify resistance genes for tetracycline, chloramphenicol, and aminoglycosides.
• The microarray assay enables the simultaneous detection of Brucella spp. and its antibiotic resistance genetic profile.
• Long oligonucleotide microarray probes were optimized for specific diagnosis.

Conclusions:

• The developed microarray assay offers a practical and efficient method for diagnosing Brucellosis.
• This molecular approach facilitates the identification of antibiotic resistance, aiding in targeted treatment decisions.
• Microarray technology provides a versatile platform for comprehensive bacterial diagnostics.