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Identification and Characterization of Follistatin-Related Protein-1 Involved in the Regulation of Chinese Perch
1Department of Bioengineering and Environmental Science, Changsha University, Changsha, Hunan 410003, China. jzhang@ccsu.edu.cn.
Abstract:
Follistatin-related protein (FSRP), which belongs to a member of the follistatin family, has been postulated to be a new negative regulator of myostatin (MSTN) and is involved in muscle development. In this study, we cloned the complete cDNA sequence of FSRP-1 from Chinese perch muscle. FSRP-1 mRNA was highly expressed in the fast muscle and brain tissue of adult fish. The expression of FSRP-1 rapidly increased at 90 days post hatching (dph) in the fast muscle of Chinese perch. Furthermore, to investigate the roles of FSRP-1 in muscle growth, we constructed a FSRP- 1 expression vector and isolated FSRP-1 fusion protein. The purified FSRP-1 fusion protein was injected into the muscle tissues of the Chinese perch (90 dph). The results showed that the number of muscle fibers and the satellite cell activation frequency were increased in the FSRP-1 treatment group. In addition, the myostatin (MSTN) expression was significantly decreased upon the FSRP-1 treatment. Collectively, the results suggest a possible mechanism of the FSRP-1 for inhibiting MSTN activity and enhancing muscle growth and renewal in vivo, and it may provide an applicable implication for the defected muscle repairing and regeneration.
Insights
Follistatin-related protein-1 (FSRP-1) enhances muscle growth in Chinese perch by inhibiting myostatin (MSTN) expression. This suggests FSRP-1
Area of Science:
- Aquaculture
- Fish biology
- Muscle physiology
Background:
- Follistatin-related protein (FSRP) is a potential regulator of muscle development.
- Myostatin (MSTN) is a key inhibitor of muscle growth.
- Understanding FSRP-1's role in fish muscle is crucial for aquaculture.
Purpose of the Study:
- To clone and characterize the FSRP-1 gene in Chinese perch.
- To investigate the effect of FSRP-1 on muscle growth and MSTN expression in vivo.
- To explore FSRP-1's potential in muscle regeneration.
Main Methods:
- Cloning of FSRP-1 cDNA from Chinese perch muscle.
- Quantitative analysis of FSRP-1 mRNA expression in different tissues and developmental stages.
- In vivo administration of purified FSRP-1 fusion protein to juvenile fish.
- Assessment of muscle fiber number, satellite cell activation, and MSTN expression.
Main Results:
- FSRP-1 mRNA was highly expressed in fast muscle and brain, with increased expression at 90 days post-hatching.
- Injection of FSRP-1 protein increased muscle fiber number and satellite cell activation.
- FSRP-1 treatment significantly decreased MSTN expression in Chinese perch muscle.
Conclusions:
- FSRP-1 inhibits MSTN activity, promoting muscle growth and regeneration in Chinese perch.
- FSRP-1 may serve as a therapeutic agent for muscle-related disorders.
- This study provides insights into FSRP-1's role in fish muscle development and repair.
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