ACA-Pro: calibration protocol for quantitative diffuse reflectance spectroscopy. Validation on contact and noncontact...

Area of Science:

• Biomedical Optics
• Spectroscopy
• Medical Imaging

Background:

• Diffuse reflectance spectroscopy (DRSsr) systems require accurate calibration to quantify tissue optical properties.
• Instrumental variations and experimental conditions can affect the reliability of DRSsr measurements.

Purpose of the Study:

• To develop and validate an adaptive calibration algorithm and protocol (ACA-Pro) for spatially resolved DRSsr.
• To enable robust quantification of absorption and scattering properties in biological tissues.

Main Methods:

• Developed ACA-Pro using a Monte Carlo-based look-up-table approach.
• Utilized a calibration reference base with various intralipid phantoms.
• Incorporated a solid material measurement for accounting experimental variations.
• Validated ACA-Pro in contact and noncontact probe-based DRSsr systems.

Main Results:

• ACA-Pro effectively corrects instrumental response in DRSsr systems.
• A single calibration reference base is applicable across different experiments.
• Validated robustness with a probe-free setup using a CCD detector.

Conclusions:

• ACA-Pro provides a reliable method for quantifying tissue optical properties.
• The protocol enhances the accuracy and consistency of DRSsr measurements.
• Demonstrated the adaptability and robustness of ACA-Pro for future applications.