Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Site-Targeted Drug Delivery Systems: Polymeric Carriers01:24

Site-Targeted Drug Delivery Systems: Polymeric Carriers

83
Polymeric carriers enhance targeted drug delivery by increasing efficacy while minimizing off-target effects. These carriers comprise a biodegradable polymeric backbone integrated with functional elements that enable targeting, improve physicochemical properties, and regulate drug release.Targeting MechanismsThe targeting ability of polymeric carriers is mediated by a homing device, which is a molecular recognition component designed to selectively bind to specific tissues or cells. Monoclonal...
83
Complexation Equilibria: The Chelate Effect01:19

Complexation Equilibria: The Chelate Effect

1.5K
In complexation reactions, metal atoms or cations interact with ligands to form donor-acceptor adducts called metal complexes. Ligands that bind through one donor site are monodentate, ligands with two donor sites are bidentate, and those with more than two donor sites are polydentate ligands. For example, ethylene diamine is a bidentate ligand that binds through two nitrogen donor atoms, forming a five-membered ring. EDTA is a polydentate ligand that binds through four oxygen and two nitrogen...
1.5K
Complexometric Titration: Ligands00:43

Complexometric Titration: Ligands

2.6K
Different monodentate and polydentate ligands are used as complexing agents in complexometric titration reactions. The formation of complexes by mono- and bidentate ligands involves two or more intermediate steps, limiting their use as complexing agents. In comparison, polydentate ligands can form complexes with metal ions in a single-step process, facilitating sharper end points. This means polydentate ligands, such as amino carboxylic acid derivatives, are most commonly employed in...
2.6K
EDTA: Chemistry and Properties01:22

EDTA: Chemistry and Properties

3.8K
Polydentate ligands are most widely used in complexometric titrations because they form more stable complexes with the metal ions than mono- or bidentate ligands due to the chelate effect. Examples of polydentate ligands are ethylenediaminetetraacetic acid (EDTA), crown ethers, and cryptands. The most important feature of optimal polydentate ligands is the ability to form 1:1 complexes in a single-step process. Amino carboxylic acid derivatives are frequently used as complexing agents. EDTA is...
3.8K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Influence of Side-Chain Molecular Features on Aqueous Coacervation of Multifunctional Homopolypeptides.

Polymer science & technology (Washington, D.C.)·2025
Same author

Shape Transformation of Poly(l-methionine sulfoxide)-<i>b</i>-poly(dehydroalanine) Vesicles.

ACS macro letters·2025
Same author

Peptide Materials.

Biomacromolecules·2025
Same author

Triggered Inversion of Dual Responsive Diblock Copolypeptide Vesicles.

Journal of the American Chemical Society·2025
Same author

Switchable Coacervate Formation via Amino Acid Functionalization of Poly(dehydroalanine).

Biomacromolecules·2024
Same author

Sulfur Switches for Responsive Peptide Materials.

Accounts of chemical research·2024

Related Experiment Video

Updated: Mar 19, 2026

Anionic Polymerization of an Amphiphilic Copolymer for Preparation of Block Copolymer Micelles Stabilized by &#960;-&#960; Stacking Interactions
10:53

Anionic Polymerization of an Amphiphilic Copolymer for Preparation of Block Copolymer Micelles Stabilized by π-π Stacking Interactions

Published on: October 10, 2016

14.7K

Protein Complexation and pH Dependent Release Using Boronic Acid Containing PEG-Polypeptide Copolymers.

Graciela E Negri1, Timothy J Deming1,2

  • 1Department of Chemistry and Biochemistry, University of California, Los Angeles, 607 Charles E Young Dr. E, Los Angeles, CA, 90095-1600, USA.

Macromolecular Bioscience
|June 11, 2016
PubMed
Summary

New polypeptide copolymers with Wulff-type phenylboronic acid (WBA) show pH-responsive carbohydrate binding. These WBA-modified copolymers form stable complexes with proteins, releasing them under acidic conditions for potential intracellular delivery.

Keywords:
block copolymerboronic acidpolypeptideprotein delivery

More Related Videos

Combinatorial Synthesis of and High-throughput Protein Release from Polymer Film and Nanoparticle Libraries
10:58

Combinatorial Synthesis of and High-throughput Protein Release from Polymer Film and Nanoparticle Libraries

Published on: September 6, 2012

10.8K
Assembly and Characterization of Polyelectrolyte Complex Micelles
08:44

Assembly and Characterization of Polyelectrolyte Complex Micelles

Published on: March 2, 2020

11.6K

Related Experiment Videos

Last Updated: Mar 19, 2026

Anionic Polymerization of an Amphiphilic Copolymer for Preparation of Block Copolymer Micelles Stabilized by &#960;-&#960; Stacking Interactions
10:53

Anionic Polymerization of an Amphiphilic Copolymer for Preparation of Block Copolymer Micelles Stabilized by π-π Stacking Interactions

Published on: October 10, 2016

14.7K
Combinatorial Synthesis of and High-throughput Protein Release from Polymer Film and Nanoparticle Libraries
10:58

Combinatorial Synthesis of and High-throughput Protein Release from Polymer Film and Nanoparticle Libraries

Published on: September 6, 2012

10.8K
Assembly and Characterization of Polyelectrolyte Complex Micelles
08:44

Assembly and Characterization of Polyelectrolyte Complex Micelles

Published on: March 2, 2020

11.6K

Area of Science:

  • Biomaterials Science
  • Polymer Chemistry
  • Cell Biology

Background:

  • Developing effective intracellular drug delivery systems is crucial for targeted therapies.
  • pH-responsive polymers offer potential for controlled release in specific cellular compartments.
  • Glycosylated proteins require specialized carriers for efficient and safe delivery.

Purpose of the Study:

  • To synthesize and characterize novel poly(L-lysine)-b-poly(ethylene glycol) copolypeptides.
  • To incorporate Wulff-type phenylboronic acid (WBA) groups for enhanced pH-responsive carbohydrate binding.
  • To evaluate the potential of these WBA-modified copolymers as carriers for intracellular protein delivery.

Main Methods:

  • Synthesis of poly(L-lysine)-b-poly(ethylene glycol) block copolymers.
  • Modification of lysine side-chain amine groups with Wulff-type phenylboronic acid (WBA).
  • Formation and characterization of nanoscale complexes with glycosylated proteins.
  • Assessment of complex stability and dissociation under varying pH conditions.

Main Results:

  • Successfully prepared WBA-modified polypeptide copolymers.
  • Demonstrated pH-responsive carbohydrate binding properties of the WBA groups.
  • Observed formation of stable nanoscale complexes with glycosylated proteins at physiological pH.
  • Confirmed dissociation and release of glycoproteins under acidic conditions (pH mimicking endosomes/lysosomes).

Conclusions:

  • WBA-modified polypeptide copolymers exhibit promising pH-responsive behavior for carbohydrate binding.
  • These copolymers can form stable complexes with glycosylated proteins, releasing them in acidic intracellular environments.
  • WBA-modified polypeptide copolymers represent a degradable carrier system with potential for intracellular protein delivery applications.