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Related Experiment Video

Updated: Mar 19, 2026

Computed Tomography-guided Time-domain Diffuse Fluorescence Tomography in Small Animals for Localization of Cancer Biomarkers

Computed Tomography-guided Time-domain Diffuse Fluorescence Tomography in Small Animals for Localization of Cancer Biomarkers

Published on: July 17, 2012

Small-Animal Imaging Using Diffuse Fluorescence Tomography.

Scott C Davis¹, Kenneth M Tichauer²

¹Thayer School of Engineering, Dartmouth College, 14 Engineering Drive, Hanover, NH, 03755, USA. scott.c.davis@dartmouth.edu.

Methods in Molecular Biology (Clifton, N.J.)

|June 11, 2016

Summary

This summary is machine-generated.

Diffuse fluorescence tomography (DFT) images fluorescent tracers in tissue, enabling functional parameter recovery. This guide simplifies using multimodal MRI-DFT for receptor-targeted imaging in small animals.

Keywords:

Anatomical priors Cancer Diffuse optics Fluorescence Image reconstruction MRI Molecular imaging Mutlimodal imaging NIRFAST

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Area of Science:

Biomedical imaging
Molecular imaging
Preclinical research

Background:

Diffuse fluorescence tomography (DFT) images fluorescent tracers in vivo.
DFT enables recovery of functional parameters like enzymatic activity and gene expression.
Multimodal systems combining DFT with conventional imaging are complex.

Purpose of the Study:

To provide a step-by-step guide for using MRI-DFT imaging.
To demonstrate imaging of a receptor-targeted tracer in small animals.
To simplify the deployment of complex multimodal imaging systems.

Main Methods:

Utilizing Magnetic Resonance Imaging (MRI) combined with Diffuse Fluorescence Tomography (DFT).
Employing a receptor-targeted fluorescent tracer.
Applying the methodology in small animal models.

Main Results:

Successful spatial distribution imaging of the fluorescent tracer.
Demonstration of multimodal MRI-DFT application.
Facilitation of functional parameter recovery.

Conclusions:

MRI-DFT is a powerful tool for preclinical molecular imaging.
This guide simplifies the complex process of multimodal DFT deployment.
The methodology aids in understanding receptor-targeted tracer dynamics.