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Optical waveguide lightmode spectroscopy technique-based immunosensor development for aflatoxin B1 determination in

Krisztina Majer-Baranyi1, Zsolt Zalán1, Mária Mörtl2

  • 1Food Science Research Institute, National Agricultural Research and Innovation Centre, Herman Ottó út 15, H-1022 Budapest, Hungary.

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|June 11, 2016
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Summary

A new Optical Waveguide Lightmode Spectroscopy (OWLS) immunosensor offers a rapid, label-free method for detecting aflatoxin B1 in paprika. This OWLS technique shows excellent correlation with HPLC and ELISA, highlighting its potential for quick food safety analysis.

Keywords:
Aflatoxin B1ELISAImmunosensorOWLSSpice paprika

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Area of Science:

  • Analytical Chemistry
  • Food Science
  • Biosensing Technology

Background:

  • Aflatoxin B1 (AFB1) is a toxic contaminant found in food products, necessitating accurate detection methods.
  • Traditional methods for AFB1 detection can be time-consuming and require complex sample preparation.
  • Developing rapid and sensitive detection techniques is crucial for ensuring food safety, particularly in spices like paprika.

Purpose of the Study:

  • To develop and optimize an Optical Waveguide Lightmode Spectroscopy (OWLS) immunosensor for label-free detection of aflatoxin B1 (AFB1).
  • To compare the analytical performance of the OWLS immunosensor with established methods like High-Performance Liquid Chromatography (HPLC) and Enzyme-Linked Immunosorbent Assay (ELISA).
  • To evaluate the effectiveness of the QuEChERS sample preparation method in conjunction with ELISA for AFB1 detection in paprika.

Main Methods:

  • Utilized Optical Waveguide Lightmode Spectroscopy (OWLS) in a competitive immunoassay format for label-free AFB1 detection.
  • Employed High-Performance Liquid Chromatography (HPLC) and Enzyme-Linked Immunosorbent Assay (ELISA) as reference methods for AFB1 quantification.
  • Assessed the QuEChERS (Quick, Easy, Cheap, Effective, Rugged, and Safe) method for sample preparation prior to ELISA analysis, comparing it with traditional solvent extraction and immunoaffinity clean-up.

Main Results:

  • The developed OWLS immunosensor demonstrated excellent correlation (regression coefficients > 0.94) with both HPLC and ELISA methods for AFB1 detection in paprika.
  • The OWLS technique provided label-free, real-time detection, offering advantages in speed and simplicity.
  • The QuEChERS method showed comparable results to traditional extraction methods when used with ELISA, indicating its applicability.

Conclusions:

  • The competitive OWLS immunosensor is a viable and promising tool for the rapid, label-free determination of aflatoxin B1 in paprika.
  • The OWLS method offers a reliable alternative to conventional techniques, with high accuracy and excellent correlation.
  • This technology has significant potential for routine food safety monitoring and quality control of spice products.