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A Method for Lineage Tracing of Corneal Cells Using Multi-color Fluorescent Reporter Mice
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Quantitative lineage tracing strategies to resolve multipotency in tissue-specific stem cells
Aline Wuidart1, Marielle Ousset1, Steffen Rulands2
1Institut de Recherche Interdisciplinaire en Biologie Humaine et Moléculaire (IRIBHM), Université Libre de Bruxelles, Brussels B-1070, Belgium;
Genes & Development
|June 11, 2016
Summary
New lineage tracing methods reveal stem cell (SC) dynamics. Prostate development involves multipotent SCs, while mammary glands use unipotent SCs for development and remodeling.
Area of Science:
- Developmental Biology
- Stem Cell Biology
- Regenerative Medicine
Background:
- Lineage tracing is crucial for studying stem cell (SC) fate and dynamics.
- Existing Cre driver methods present challenges due to dynamic, temporal, and heterogeneous expression, complicating data interpretation.
Purpose of the Study:
- To develop novel methods for robust lineage tracing and stem cell fate assessment.
- To differentiate between multipotent and unipotent stem cell contributions in tissue development and homeostasis.
Main Methods:
- Statistical analysis of multicolor lineage tracing to confidently define multipotency.
- Saturation lineage tracing to assess the fate of all stem cells and cell flux between lineages.
Main Results:
- Prostate development originates from multipotent stem cells.
- Mammary gland development and adult tissue remodeling are mediated by unipotent stem cells.
Conclusions:
- The developed methods provide a rigorous framework for analyzing stem cell lineage relationships and fate.
- Distinguishes distinct stem cell behaviors in prostate and mammary gland development and regeneration.
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