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Updated: Mar 19, 2026
Synthesis of Wavelength-shifting DNA Hybridization Probes by Using Photostable Cyanine Dyes
Published on: July 6, 2016
Three-Dimensional Control of DNA Hybridization by Orthogonal Two-Color Two-Photon Uncaging
Manuela A H Fichte1, Xenia M M Weyel1, Stephan Junek2
1Institute of Organic Chemistry and Chemical Biology, Goethe-University Frankfurt, Max-von-Laue-Strasse 9, 60438, Frankfurt am Main, Germany.
Abstract:
We successfully introduced two-photon-sensitive photolabile groups ([7-(diethylamino)coumarin-4-yl]methyl and p-dialkylaminonitrobiphenyl) into DNA strands and demonstrated their suitability for three-dimensional photorelease. To visualize the uncaging, we used a fluorescence readout based on double-strand displacement in a hydrogel and in neurons. Orthogonal two-photon uncaging of the two cages is possible, thus enabling complex scenarios of three-dimensional control of hybridization with light.
