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A chemiluminescent assay for measuring avermectin binding sites
J M Schaeffer1, J H Stiffey, H Mrozik
1Merck Sharp & Dohme Research Laboratories, Rahway, New Jersey 07065.
Analytical Biochemistry
|March 1, 1989
Abstract:
Avermectins are potent, broad spectrum anthelmintics. We describe the synthesis of a biologically active chemiluminescent compound, 22,23-dihydroavermectin B1a, 4"-[4-[[4-[ethyl(1,2,3,4-tetrahydro-1,4-dioxo-6- phthalazinyl)amino]butyl]amino]-4-oxobutanoate] (ivermectin-luminol). This compound retains its anthelmintic properties and provides an extremely sensitive nonradioactive probe to study avermectin binding sites.